Wei Huanhuan, Wang Zefeng
Key Laboratory of Computational Biology, MPG-CAS Partner Institute of Computational Biology, Shanghai, China.
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Wiley Interdiscip Rev RNA. 2015 Nov-Dec;6(6):597-613. doi: 10.1002/wrna.1296. Epub 2015 Aug 28.
With generations of efforts to understand RNA functions in diverse cellular processes, RNA-binding proteins (RBPs) have emerged to be one of the central players in regulating RNA-related pathways. RBPs control almost all aspects of RNA processing via recognizing their RNA target(s). Most of these proteins have a modular configuration, with one or more RNA-binding domain for target recognition and various functional modules to affect the metabolism and biological functions of RNA. Thus, engineering RNA-binding factors with customized specificity and function is extremely useful in biological and medical research. In this review, we discuss the current advances in engineering RBPs that specifically bind to diverse targets, with emphasis on the design strategies and their applications as new biological tools in various aspects of RNA metabolism and function.
经过几代人对RNA在各种细胞过程中功能的研究，RNA结合蛋白（RBPs）已成为调节RNA相关途径的核心参与者之一。RBPs通过识别其RNA靶标来控制RNA加工的几乎所有方面。这些蛋白质中的大多数具有模块化结构，具有一个或多个用于靶标识别的RNA结合结构域以及各种影响RNA代谢和生物学功能的功能模块。因此，设计具有定制特异性和功能的RNA结合因子在生物学和医学研究中非常有用。在这篇综述中，我们讨论了工程化RBPs以特异性结合各种靶标的当前进展，重点是设计策略及其作为RNA代谢和功能各个方面新生物工具的应用。
