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猩红色虎蛾(Callimorpha dominula)(昆虫纲:鳞翅目:灯蛾科)线粒体全基因组的特征分析

Characterization of the complete mitochondrial genome of the Scarlet Tiger moth Callimorpha dominula (Insecta: Lepidoptera: Arctiidae).

作者信息

Peng Xiao-Yi, Duan Xiao-Yu, Qiang Yi

机构信息

a Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, College of Life Sciences, Shaanxi Normal University , Xi'an , PR China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3749-50. doi: 10.3109/19401736.2015.1079888. Epub 2015 Sep 2.

DOI:10.3109/19401736.2015.1079888
PMID:26329289
Abstract

The complete mitochondrial genome of the Scarlet Tiger moth Callimorpha dominula (Insecta: Lepidoptera: Arctiidae) has been reconstructed from the whole-genome Illumina sequencing data. This circular genome is 15 496 bp in size, and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A + T-rich D-loop or control region. Most PCGs are initiated with the ATN codons, except for COX1 with the unusual CGA as its initiation codon. Four PCGs (COX1, COX2, ND3, and ND4) are terminated with incomplete codon T, ND4L uses TAG as its termination codon, while all the other eight PCGs employ the usual ATN codons. The nucleotide composition is highly asymmetric (40.1% A, 40.9% T, 7.6% G, and 11.4% C) with an overall A + T content of 81.0%. The phylogenetic analysis based on the neighbor-joining (NJ) method suggests that C. dominula is more phylogenetically related to its confamilial counterparts than to those from other families.

摘要

通过全基因组Illumina测序数据重建了红虎蛾(Callimorpha dominula)(昆虫纲:鳞翅目:灯蛾科)的完整线粒体基因组。这个环状基因组大小为15496 bp,包含13个蛋白质编码基因(PCGs)、22个转运RNA(tRNAs)、两个核糖体RNA(rRNAs)和一个富含A+T的D环或控制区。大多数PCGs以ATN密码子起始,除了COX1以不寻常的CGA作为起始密码子。四个PCGs(COX1、COX2、ND3和ND4)以不完全密码子T终止,ND4L使用TAG作为其终止密码子,而其他八个PCGs则使用常见的ATN密码子。核苷酸组成高度不对称(A占40.1%、T占40.9%、G占7.6%、C占11.4%),A+T总含量为81.0%。基于邻接法(NJ)的系统发育分析表明,红虎蛾与其同科物种在系统发育上的关系比与其他科的物种更为密切。

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