Liao W, Gu C, Huang A, Yao J, Sun R
Department of General Surgery, Jinshan Hospital, Fudan University, No. 1508, Longhang Road, Shanghai, 201508, People's Republic of China.
Clin Transl Oncol. 2016 May;18(5):449-56. doi: 10.1007/s12094-015-1388-6. Epub 2015 Sep 2.
To explore the role of miR-33b in colorectal cancer (CRC) and the correlation between its expression and prognosis.
The expressions of miR-33b between CRC tissues and normal tissues were measured by real-time PCR. The effects of miR-33b on cell proliferation and cell cycle progression were detected by MTT assay, colony formation assay and flow cytometry. The potential regulations of miR-33b on multiple genes expression were verified by Western blot. Furthermore, the association of miR-33b with CRC clinicopathologic features and prognosis was analyzed by Chi-squared test and Kaplan-Meier tests.
MiR-33b was downregulated in CRC compared with normal colorectal samples and miR-33b inhibited tumor cell growth and induced cell cycle arrest. Western blot assays and correlation analysis showed that miR-33b could regulate multiple growth-related genes. Moreover, the expression of miR-33b was associated with TNM stage and tumor size, and CRC patients with high miR-33b expression had a better prognosis.
Our data suggest that miR-33b functions as a tumor suppressor gene in CRC through regulating cell proliferation and cell cycle.
探讨miR-33b在结直肠癌（CRC）中的作用及其表达与预后的相关性。
采用实时荧光定量PCR检测CRC组织和正常组织中miR-33b的表达。通过MTT法、集落形成试验和流式细胞术检测miR-33b对细胞增殖和细胞周期进程的影响。通过蛋白质印迹法验证miR-33b对多个基因表达的潜在调控作用。此外，采用卡方检验和Kaplan-Meier检验分析miR-33b与CRC临床病理特征及预后的相关性。
与正常结直肠样本相比，CRC中miR-33b表达下调，且miR-33b抑制肿瘤细胞生长并诱导细胞周期停滞。蛋白质印迹分析和相关性分析表明，miR-33b可调控多个与生长相关的基因。此外，miR-33b的表达与TNM分期和肿瘤大小相关，miR-33b高表达的CRC患者预后较好。
我们的数据表明，miR-33b在CRC中作为肿瘤抑制基因发挥作用，通过调节细胞增殖和细胞周期来实现。
