Wang Qi-Bao, Sun Lan-Ying, Gong Zuo-de, Du Yi
Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, 250001, China.
Dental Implant Center, Jinan Stomatological Hospital, Jinan, Shandong, 250001, China.
Inflammation. 2016 Feb;39(1):237-242. doi: 10.1007/s10753-015-0243-9.
Veratric acid, one of the major benzoic acid isolated from vegetables and fruits, has been reported to have anti-inflammatory activity. The purpose of this study was to investigate the anti-inflammatory effects of veratric acid on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). HGFs were pretreated with veratric acid 1 h before LPS stimulation. The productions of IL-6 and IL-8 were detected by ELISA. The expression of iNOS, COX-2, PI3K, AKT, and NF-κB were detected by western blotting. The results showed that veratric acid inhibited LPS-induced IL-6 and IL-8 production, as well as iNOS and COX-2 expression. Veratric acid also inhibited LPS-induced NF-κB activation. In addition, veratric acid was found to suppress LPS-induced PI3K and AKT phosphorylation. In conclusion, the anti-inflammatory mechanism of veratric acid is due to its ability to inhibit PI3K/Akt/NF-κB signaling pathway.
藜芦酸是从蔬菜和水果中分离出的主要苯甲酸之一，据报道具有抗炎活性。本研究的目的是探讨藜芦酸对脂多糖（LPS）诱导的人牙龈成纤维细胞（HGFs）炎症反应的抗炎作用。在LPS刺激前1小时用藜芦酸预处理HGFs。通过ELISA检测IL-6和IL-8的产生。通过蛋白质印迹法检测诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、磷脂酰肌醇-3激酶（PI3K）、蛋白激酶B（AKT）和核因子κB（NF-κB）的表达。结果表明，藜芦酸抑制LPS诱导的IL-6和IL-8产生，以及iNOS和COX-2表达。藜芦酸还抑制LPS诱导的NF-κB活化。此外，发现藜芦酸可抑制LPS诱导的PI3K和AKT磷酸化。总之，藜芦酸的抗炎机制是由于其抑制PI3K/Akt/NF-κB信号通路的能力。
