Zhang Na, Lv Hui, Shi Bo-Hong, Hou Xin, Xu Xin
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Shandong, China; Department of Stomatology, The People's Hospital of Rizhao, Shandong, China.
Department of Stomatology, The People's Hospital of Rizhao, Shandong, China.
Microb Pathog. 2017 Sep;110:135-139. doi: 10.1016/j.micpath.2017.06.021. Epub 2017 Jun 23.
The aim of this study was to clarify the anti-inflammatory effects and its molecular mechanism of glycyrrhizin on LPS-stimulated human gingival fibroblasts (HGFs), which will be of benefit for periodontitis treatment. An MTT assay was performed to assess the effects of glycyrrhizin on cellular viability. The levels of IL-6 and IL-8 were measured by ELISA. The expression of iNOS, COX-2, NF-κB, and LXRα were detected by western blot analysis. The results showed that glycyrrhizin significantly inhibited LPS-induced IL-6 and IL-8 production, as well as COX-2 and iNOS expression. LPS-induced NF-κB activation in HGFs was also inhibited by treatment of glycyrrhizin. Furthermore, glycyrrhizin increased the expression of LXRα in a concentration-dependent manner. In addition, the inhibition of glycyrrhizin on IL-6 and IL-8 production was reversed by LXRα inhibitor GGPP. In conclusion, these results indicated that glycyrrhizin exhibited its anti-inflammatory effects in HGFs by activating LXRα.
本研究旨在阐明甘草酸对脂多糖（LPS）刺激的人牙龈成纤维细胞（HGFs）的抗炎作用及其分子机制，这将有助于牙周炎的治疗。采用MTT法评估甘草酸对细胞活力的影响。通过酶联免疫吸附测定（ELISA）检测白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的水平。通过蛋白质免疫印迹分析检测诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、核因子κB（NF-κB）和肝X受体α（LXRα）的表达。结果表明，甘草酸显著抑制LPS诱导的IL-6和IL-8产生，以及COX-2和iNOS表达。甘草酸处理也抑制了LPS诱导的HGFs中NF-κB激活。此外，甘草酸以浓度依赖性方式增加LXRα的表达。此外，LXRα抑制剂香叶基香叶基焦磷酸（GGPP）逆转了甘草酸对IL-6和IL-8产生的抑制作用。总之，这些结果表明甘草酸通过激活LXRα在HGFs中发挥其抗炎作用。
