Su Shulan, Duan Jinao, Chen Ting, Huang Xiaochen, Shang Erxin, Yu Li, Wei Kaifeng, Zhu Yue, Guo Jianming, Guo Sheng, Liu Pei, Qian Dawei, Tang Yuping
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine; Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
Sci Rep. 2015 Sep 2;5:13668. doi: 10.1038/srep13668.
Frankincense and myrrh are highly effective in treatment of inflammatory diseases, but lacking of thetherapy mechanisms. We undertook this study to evaluate the effects on Adjuvant-induced Arthritis(AIA) rats and to explore the underlying mechanisms by analyzing the metabolic profiling and signalingpathway evaluated by expression of inflammatory cytokines, c-jun and c-fos and corresponding phosphorylationlevels. [corrected]. The results stated the elevated expression levels of TNFα, PGE2, IL-2, NO, and MDA in serum and swelling paw of AIA rats were significantly decreased after treatment, which exerted more remarkable inhibitive effects of combined therapy. The metbolic profiling of plasma and urine were clearly improved and twenty-one potential biomarkers were identified. Moreover, the inhibited effects of five bioactive components on cytokine transcription in PHA stimulated-PBMC showed the MAPK pathway might account for this phenomenon with considerable reduction in phosphorylated forms of all the three MAPK (ERK1/2, p38 and JNK) and down regulation of c-jun and c-fos.
乳香和没药在治疗炎症性疾病方面非常有效，但缺乏治疗机制。我们进行了这项研究，以评估其对佐剂性关节炎（AIA）大鼠的影响，并通过分析代谢谱以及通过炎症细胞因子、c-jun和c-fos的表达及相应磷酸化水平评估的信号通路来探索潜在机制。[已修正]。结果表明，治疗后AIA大鼠血清和肿胀爪中TNFα、PGE2、IL-2、NO和MDA的表达水平升高显著降低，联合治疗的抑制作用更为显著。血浆和尿液的代谢谱明显改善，并鉴定出21种潜在生物标志物。此外，五种生物活性成分对PHA刺激的PBMC中细胞因子转录的抑制作用表明，MAPK通路可能是导致这种现象的原因，所有三种MAPK（ERK1/2、p38和JNK）的磷酸化形式显著降低，c-jun和c-fos下调。
