Storms Zachary J, Sauvageau Dominic
Department of Chemical and Materials Engineering, University of Alberta, 9107-116th Street, Edmonton, Alberta, Canada T6G 2V4.
Department of Chemical and Materials Engineering, University of Alberta, 9107-116th Street, Edmonton, Alberta, Canada T6G 2V4.
Virology. 2015 Nov;485:355-62. doi: 10.1016/j.virol.2015.08.007. Epub 2015 Aug 29.
The process of a bacteriophage attaching to its host cell is a combination of physical diffusion, biochemical surface interactions, and reaction-induced conformational changes in receptor proteins. Local variations in the physico-chemical properties of the medium, the phage׳s mode of action, and the physiology of the host cell also all influence adsorption kinetics. These characteristics can affect a specific phage׳s binding capabilities and the susceptibility of the host cell to phage attack. Despite the complexity of this process, describing adsorption kinetics of a population of bacteriophages binding to a culture of cells has been accomplished with relatively simple equations governed by the laws of mass-action. Many permutations and modifications to the basic set of reactions have been suggested through the years. While no single solution emerges as a universal answer, this review provides the fundamentals of current phage adsorption modeling and will guide researchers in the selection of valid, appropriate models.
噬菌体附着于宿主细胞的过程是物理扩散、生化表面相互作用以及受体蛋白中反应诱导的构象变化的结合。培养基理化性质的局部变化、噬菌体的作用方式以及宿主细胞的生理学特性也都会影响吸附动力学。这些特性会影响特定噬菌体的结合能力以及宿主细胞对噬菌体攻击的敏感性。尽管这个过程很复杂，但通过遵循质量作用定律的相对简单的方程，已经实现了对一群噬菌体与细胞培养物结合的吸附动力学的描述。多年来，人们提出了对基本反应集的许多排列和修改。虽然没有一个单一的解决方案能成为通用答案，但本综述提供了当前噬菌体吸附建模的基本原理，并将指导研究人员选择有效、合适的模型。
