miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma.

OBJECTIVES

MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour.

MATERIALS AND METHODS

qRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372's target gene.

RESULTS

We demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3'-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression.

CONCLUSIONS

Our data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.