Luke Chung Sze, Selimkhanov Jangir, Baumgart Leo, Cohen Susan E, Golden Susan S, Cookson Natalie A, Hasty Jeff
Department of Bioengineering, University of California, San Diego , La Jolla, California 92093, United States.
Division of Biological Sciences, University of California, San Diego , La Jolla, California 92093, United States.
ACS Synth Biol. 2016 Jan 15;5(1):8-14. doi: 10.1021/acssynbio.5b00094. Epub 2015 Sep 15.
Culturing cells in microfluidic "lab-on-a-chip" devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.
在微流控“芯片实验室”设备中培养细胞用于延时显微镜观察，已成为研究生物系统动态的一项有价值的工具。尽管微流控技术已应用于培养和监测多种细菌及真核生物，但蓝细菌和真核微藻存在一些挑战，使其难以在微流控环境中培养。在此，我们展示了一种可定制的设备，用于对三种特征明确的蓝细菌和微藻菌株进行长期培养和成像。该平台相对于琼脂糖垫具有多个优势，并展现出在精确控制的动态环境中长时间获取蓝细菌和藻类高质量单细胞基因表达数据的巨大潜力。
