Park Sang-Oh, Park Byung-Sung
Institute of Animal Resources, Kangwon National University, Chuncheon, Gangwondo 200-701, Korea.
J Biosci. 2015 Sep;40(3):513-20. doi: 10.1007/s12038-015-9540-6.
The main objective of this study was to investigate whether orally administered Korean grain larvae ethanol extract (GLE) had a bifidogenic effect in normal rats. Male Sprague-Dawley rats were divided into a negative control group (CO) and GLE orally administered (5.0, 7.0 and 9.0 mg/100 g body weight) groups. Thymus and spleen weights dosedependently increased by 128.58 percent and 128.58 percent, respectively, but abdominal fat decreased by 19.18 percent after GLE administration compared with that in the CO group (p less than 0.05). Serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose decreased by 30.26 percent, 7.33 percent, 27.20 percent, and 6.96 percent, respectively, whereas highdensity lipoprotein cholesterol increased by 129.93 percent in the GLE groups compared with those in the CO group (p less than 0.05). IgG, IgM, IgA in the GLE groups increased 203.68 percent, 181.41 percent, and 238.25 percent, respectively, compared to that in the CO group (p less than 0.05). Bifidobacteria and Lactobacillus increased by 115.74 percent and 144.28 percent, whereas Bacteroides, Clostridium, Escherichia, and Streptococcus decreased by 17.37 percent, 17.46 percent, 21.25 percent, and 19.16 percent, respectively, in the GLE groups compared with those in the CO group (p less than 0.05). Total organic acids, acetic acid, and propionic acid increased by 151.40 percent, 188.09 percent, and 150.17 percent, whereas butyric acid and valeric acid decreased by 40.65 percent and 49.24 percent, respectively, in the GLE groups as compared with those in the CO group (p less than 0.05). These results suggest that Korean GLE improves the bifidogenic effect by increasing cecal organic acids and modulating gut microflora via a selective increase in Bifidobacterium in normal rats.
本研究的主要目的是调查口服韩国谷物幼虫乙醇提取物（GLE）对正常大鼠是否具有双歧杆菌增殖效应。雄性斯普拉格-道利大鼠被分为阴性对照组（CO）和口服GLE组（5.0、7.0和9.0毫克/100克体重）。与CO组相比，GLE给药后胸腺和脾脏重量分别剂量依赖性增加128.58%和128.58%，但腹部脂肪减少19.18%（p<0.05）。GLE组血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇和葡萄糖分别降低30.26%、7.33%、27.20%和6.96%，而高密度脂蛋白胆固醇增加129.93%（p<0.05）。与CO组相比，GLE组的IgG、IgM、IgA分别增加203.68%、181.41%和238.25%（p<0.05）。与CO组相比，GLE组双歧杆菌和乳酸杆菌分别增加115.74%和144.28%，而拟杆菌、梭菌、大肠杆菌和链球菌分别减少17.37%、17.46%、21.25%和19.16%（p<0.05）。与CO组相比，GLE组总有机酸、乙酸和丙酸分别增加151.40%、188.09%和150.17%，而丁酸和戊酸分别减少40.65%和49.24%（p<0.05）。这些结果表明，韩国GLE通过增加盲肠有机酸和通过正常大鼠中双歧杆菌的选择性增加来调节肠道微生物群，从而改善双歧杆菌增殖效应。
