Cardelle-Cobas Alejandra, Fernández María, Salazar Nuria, Martínez-Villaluenga Cristina, Villamiel Mar, Ruas-Madiedo Patricia, de los Reyes-Gavilán Clara G
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, Madrid 28006, Spain.
J Dairy Res. 2009 Aug;76(3):317-25. doi: 10.1017/S0022029909004063. Epub 2009 May 18.
Bifidogenic effect and stimulation of short chain fatty acid (SCFA) production by fractions of oligosaccharides with a DP> or =3 and Gal beta(1-6) linkages synthesised from lactose or lactulose by Pectinex Ultra SP-L and Lactozym 3000 L HP G were evaluated in human faecal slurries. Results were compared with those obtained for the commercial oligosaccharide mixture Vivinal-GOS. Quantitative real-time PCR showed that all galacto-oligosaccharide (GOS) fractions stimulated slightly higher bifidobacteria growth than lactose, lactulose and Vivinal-GOS. GOS fractions promoted the production of total SCFA and acetic acid in a similar way to Vivinal-GOS and more than glucose, lactose and lactulose. In conclusion, oligosaccharides derived from lactose and lactulose may represent a suitable alternative to lactulose for the design of new functional food ingredients.
在人粪便悬液中评估了由Pectinex Ultra SP-L和Lactozym 3000 L HP G从乳糖或乳果糖合成的具有DP>或=3以及Galβ(1-6)键的低聚糖级分的双歧杆菌生成效应和对短链脂肪酸(SCFA)产生的刺激作用。将结果与市售低聚糖混合物Vivinal-GOS的结果进行比较。定量实时PCR表明，所有低聚半乳糖(GOS)级分对双歧杆菌生长的刺激略高于乳糖、乳果糖和Vivinal-GOS。GOS级分促进总SCFA和乙酸产生的方式与Vivinal-GOS相似，且比葡萄糖、乳糖和乳果糖更多。总之，源自乳糖和乳果糖的低聚糖可能是乳果糖的合适替代品，可用于设计新的功能性食品成分。
