Wright K, Collins D C, Musey P I, Preedy J R
J Clin Endocrinol Metab. 1978 Nov;47(5):1092-8. doi: 10.1210/jcem-47-5-1092.
Estrone sulfate, quantitatively the most important estrogen in plasma, has previously been determined only after hydrolysis and chromatography. An antiserum raised against estrone glucosiduronate-bovine thyroglobulin was found to be suitable for the specific RIA of estrone sulfate both in plasma and urine. Plasma levels were measured after solvent extraction without hydrolysis or chromatography. The mean (+/-SE) was 972 +/- 79 pg/ml (range, 537-1581) in 15 women in the follicular phase, 1806 +/- 160 pg/ml (range, 814-3358) in 15 women in the luteal phase, and 922 +/- 62 pg/ml (range, 461-1238) in 13 men. The urinary excretion of estrone sulfate, measured after simple chromatographic separation, ranged from 0.8-7.9 micrograms/24 h in men and 5.1-18.7 micrograms/24h in nonpregnant women. This was generally one-seventh to one-half the simultaneous estrone glucosiduronate excretion rate. An approximate mean renal clearance of estrone sulfate calculated from the above values was 2.7 ml/min. The low clearance rate is taken to reflect extensive binding of estrone sulfate by plasma proteins. The splanchnic extraction of estrone sulfate measured in 6 patients undergoing hepatic vein catheterization for diagnostic purposes was 29.8 +/- 11.1%, indicating net uptake of this compound by the splanchnic area.
硫酸雌酮是血浆中数量上最重要的雌激素，此前仅在水解和色谱分析后才能测定。发现一种针对雌酮葡萄糖醛酸苷 - 牛甲状腺球蛋白产生的抗血清适用于血浆和尿液中硫酸雌酮的特异性放射免疫分析。在不进行水解或色谱分析的情况下，通过溶剂萃取后测量血浆水平。15名处于卵泡期的女性的平均值（±标准误）为972±79 pg/ml（范围为537 - 1581），15名处于黄体期的女性为1806±160 pg/ml（范围为814 - 3358），13名男性为922±62 pg/ml（范围为461 - 1238）。在简单的色谱分离后测量的硫酸雌酮尿排泄量，男性为0.8 - 7.9微克/24小时，非妊娠女性为5.1 - 18.7微克/24小时。这通常是同时期雌酮葡萄糖醛酸苷排泄率的七分之一到二分之一。根据上述值计算的硫酸雌酮的近似平均肾清除率为2.7毫升/分钟。低清除率被认为反映了硫酸雌酮与血浆蛋白的广泛结合。在6名因诊断目的接受肝静脉插管的患者中测量的硫酸雌酮的内脏提取率为29.8±11.1%，表明该化合物被内脏区域净摄取。
