Rémy-Martin A, Prost O, Nicollier M, Burnod J, Adessi G L
Clin Chem. 1983 Jan;29(1):86-9.
Estrone sulfate is quantitatively the most important estrogen in plasma. A method for its determination in human plasma is described, and the precision, accuracy, sensitivity, and specificity are defined. Free steroids were extracted from plasma with diethyl ether and steroid sulfates were isolated with use of Vlitos' reagent (methylene blue in dilute H2SO4/Na2SO4 solution). After enzymic hydrolysis, estrone was isolated by chromatography on Celite and measured by radioimmunoassay. The mean concentrations (nmol/L +/- 1 SD) of estrone sulfate were 2.51 +/- 0.90 for plasma from 13 women in follicular phase, 5.33 +/- 1.55 for 17 women in luteal phase, 0.89 +/- 0.60 for 44 postmenopausal women, and 0.96 +/- 0.43 for 24 postmenopausal women with breast cancer. Results for postmenopausal women with or without breast cancer did not differ significantly. For 13 normal men, estrone sulfate concentrations were 2.62 +/- 0.79 nmol/L, and for a group of 19 cirrhotic men the mean value was 1.43 +/- 0.95 nmol/L, significantly lower than normal.
硫酸雌酮是血浆中含量最为重要的雌激素。本文描述了一种测定人血浆中硫酸雌酮的方法，并对其精密度、准确度、灵敏度和特异性进行了定义。用乙醚从血浆中提取游离类固醇，并用维利托试剂（稀硫酸/硫酸钠溶液中的亚甲蓝）分离类固醇硫酸盐。酶水解后，通过在硅藻土上进行色谱分离法分离出雌酮，并通过放射免疫测定法进行测量。13名处于卵泡期女性的血浆中硫酸雌酮的平均浓度（nmol/L±1标准差）为2.51±0.90，17名处于黄体期女性为5.33±1.55，44名绝经后女性为0.89±0.60，24名患有乳腺癌的绝经后女性为0.96±0.43。患有或未患有乳腺癌的绝经后女性的结果无显著差异。13名正常男性的硫酸雌酮浓度为2.62±0.79 nmol/L，一组19名肝硬化男性的平均值为1.43±0.95 nmol/L，显著低于正常水平。
