From the Department of Biology, Sanandaj Branch and.
Department of Biochemistry, College of Science, Kurdistan and Research Branch, Islamic Azad University.
QJM. 2016 Jun;109(6):399-404. doi: 10.1093/qjmed/hcv149. Epub 2015 Sep 3.
Given the growing rate of patients with type 2 diabetes mellitus, uncovering the effects of gene polymorphism on diabetes pathogenesis has attracted a lot of attention. Because glucose transporter 1 is involved in glucose uptake, the polymorphism of this gene may be an important risk factor in type 2 diabetes mellitus or in the progression of diabetes complications such as diabetic nephropathy. As far as the authors are concerned, this study is the first one aiming at evaluating the probable effects of solute carrier family 2 facilitated glucose transporter member 1 (SLC2A1) HaeIII polymorphism on clinical and laboratory outcomes of Kurdish patients with type 2 diabetes mellitus.
This study was conducted involving 126 diabetic nephropathy patients and 150 diabetic patients without renal involvement. Serum levels of Cystatin C, fasting blood glucose, creatinine and urinary albumin; levels of glycated hemoglobin and estimated glomerular filtration rate were measured. Moreover, the Hae III polymorphism of SLC2A1 gene was determined by PCR-restriction fragment length polymorphism (RFLP).
The rate of CC genotype was higher (37%) in patients with diabetic nephropathy compared with controls. There were a significant correlation between the CC genotype and risk of diabetic nephropathy. There were significant correlations between genotypes, serum Cystatin C and estimated glomerular filtration rate in patients with diabetic nephropathy.
The results demonstrated the high frequency of C allele of SLC2A1 HaeIII in Kurdish patients with diabetic nephropathy. It was also found that this polymorphism is a significant risk factor for diabetic nephropathy. The effect of this polymorphism on clinical and laboratory characteristics of diabetic nephropathy patients was significant.
由于 2 型糖尿病患者的增长率不断上升，因此研究基因多态性对糖尿病发病机制的影响引起了广泛关注。葡萄糖转运蛋白 1 参与葡萄糖摄取，因此该基因的多态性可能是 2 型糖尿病或糖尿病肾病等并发症进展的重要危险因素。就作者所知，这项研究旨在评估溶质载体家族 2 促进葡萄糖转运蛋白成员 1（SLC2A1）HaeIII 多态性对库尔德 2 型糖尿病患者临床和实验室结果的可能影响。
本研究纳入了 126 例糖尿病肾病患者和 150 例无肾脏受累的糖尿病患者。测量了血清胱抑素 C、空腹血糖、肌酐和尿白蛋白；糖化血红蛋白和估计肾小球滤过率的水平。此外，通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）确定 SLC2A1 基因的 HaeIII 多态性。
与对照组相比，糖尿病肾病患者的 CC 基因型（37%）更高。CC 基因型与糖尿病肾病的风险之间存在显著相关性。糖尿病肾病患者的基因型、血清胱抑素 C 和估计肾小球滤过率之间存在显著相关性。
结果表明，库尔德糖尿病肾病患者 SLC2A1 HaeIII 的 C 等位基因频率较高。还发现该多态性是糖尿病肾病的一个重要危险因素。该多态性对糖尿病肾病患者的临床和实验室特征有显著影响。
