Feng Siqi, Yadav Shiv Kumar, Gao Fang, Yi Qijian
Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China.
Ministry of Education Key Laboratory of Child Development and Disorders; Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, PR China.
BMC Pediatr. 2015 Sep 3;15:109. doi: 10.1186/s12887-015-0424-6.
Monokines induced by interferon-gamma/Chemokine (C-X-C motif) ligand 9 (MIG/CXCL9), thymus and activation-regulated chemokine/Chemokine (C-C motif) ligand 17 (TARC/CCL17) are chemotactic factors that specifically collect and activate leukocytes, which are considered as chemoattractants of T helper cells. In the present study, we have investigated the effects of T helper type-1 (Th1) cells and T helper type-2 (Th2) cells in Kawasaki disease (KD) by determining plasma levels of MIG/CXCL9 and TARC/CCL17 and exploring the relationship between MIG/CXCL9, TARC/CCL17 levels and coronary artery lesions (CAL).
Forty-three children patients with KD and 19 healthy controls were included in this study. General characteristics were obtained from all subjects. Plasma concentrations of chemotactic factors of MIG/CXCL9 and TARC/CCL17 were measured by enzyme-linked immunosorbent assay (ELISA) for all subjects.
Plasma levels of MIG/CXCL9, TARC/CCL17, and the ratios of MIG/TARC were significantly elevated in pediatric patients with KD compared to those in the control group. There were also significantly higher levels of MIG/CXCL9, TARC/CCL17, MIG/TARC ratios and prominently lower hemoglobin (Hb) levels in KD with CAL compared to KD without coronary artery lesions (NCAL). Hb was significantly decreased and plasma MIG/CXCL9 levels had a significantly negative correlation with CRP in KD with CAL patients (KD-CAL), whereas a positive correlation of plasma MIG/CXCL 9 with WBC was observed in KD without CAL patients (KD-NCAL).
Th1 and Th2 cells may be involved in an imbalanced activation in pediatric KD patients during an acute period of the disease. Furthermore, immune lesions of vessels in KD patients may be mediated by the imbalanced activation of Th1 and Th2 cells.
γ干扰素诱导的单核因子/趋化因子（C-X-C基序）配体9（MIG/CXCL9）、胸腺和活化调节趋化因子/趋化因子（C-C基序）配体17（TARC/CCL17）是特异性募集和激活白细胞的趋化因子，被认为是辅助性T细胞的化学引诱剂。在本研究中，我们通过测定血浆中MIG/CXCL9和TARC/CCL17水平，并探讨MIG/CXCL9、TARC/CCL17水平与冠状动脉病变（CAL）之间的关系，研究了1型辅助性T细胞（Th1）和2型辅助性T细胞（Th2）在川崎病（KD）中的作用。
本研究纳入43例KD患儿和19例健康对照。获取所有受试者的一般特征。采用酶联免疫吸附测定（ELISA）法测定所有受试者血浆中MIG/CXCL9和TARC/CCL17趋化因子的浓度。
与对照组相比，KD患儿血浆MIG/CXCL9、TARC/CCL17水平及MIG/TARC比值显著升高。与无冠状动脉病变（NCAL）的KD患儿相比，有CAL的KD患儿MIG/CXCL9、TARC/CCL17水平、MIG/TARC比值也显著升高，血红蛋白（Hb）水平显著降低。在有CAL的KD患者（KD-CAL）中，Hb显著降低，血浆MIG/CXCL9水平与CRP呈显著负相关，而在无CAL的KD患者（KD-NCAL）中，血浆MIG/CXCL9与白细胞呈正相关。
Th1和Th2细胞可能参与了小儿KD患者急性期的激活失衡。此外，KD患者血管的免疫损伤可能由Th1和Th2细胞的激活失衡介导。
