Zhang Y, Verhoeff N I, Chen Z, Chen S, Wang Mei, Zhu Zhen, Ouwerkerk P B F
Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
Plant Mol Biol. 2015 Oct;89(3):229-42. doi: 10.1007/s11103-015-0357-3. Epub 2015 Sep 3.
Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator.
人们对植物中所谓的Dof锌因子的功能了解相对较少。在此，我们报告了对OsDof25的分析，并展示了其在调控水稻重要的C4光合作用基因OsC4PPDK中的功能。在瞬时表达实验中，OsDof25的过表达通过以特定方式结合到一个保守的Dof结合位点，增强了OsC4PPDK的表达，酵母和体外结合研究证实了这一点。使用启动子GUS植物以及qPCR实验进行的表达研究表明，OsDof25在包括光合和非光合器官在内的不同组织中表达，并且OsDof25的表达与OsC4PPDK基因部分重叠。来自转基因水稻功能缺失和功能获得实验的确凿证据表明，OsDof25在调控C4PPDK中发挥作用，该实验表明OsDof25的下调或过表达与OsC4PPDK表达相关，并且OsDof25具有转录激活因子的功能。
