Zou Weiying, Lin Hua, Liu Wenwen, Yang Bei, Wu Lei, Duan Limin, Ling Ping, Zhu Lingyan, Dai Qun, Zhao Lintong, Zou Ting, Zhang Dalei
Basic Medical College of Nanchang University, Nanchang, China.
Nanchang Medical School, Nanchang, China.
Acupunct Med. 2016 Apr;34(2):114-9. doi: 10.1136/acupmed-2015-010788. Epub 2015 Sep 3.
To investigate the effects of moxibustion on visceral hyperalgesia (VH) and bone marrow cell transient receptor potential vanilloid type 1 (TRPV1) and heat shock protein (HSP) 70 expression in a rat model of VH.
Mechanical colorectal distension was performed to induce VH in neonatal Sprague-Dawley rats. Eight-week-old VH rats were treated with moxibustion at acupuncture point BL25 or an ipsilateral non-acupuncture point. Abdominal withdrawal reflex (AWR) scoring and pain threshold pressure assessment were performed before and after moxibustion treatment for 7 consecutive days. The expression of TRPV1 and HSP70 in bone marrow cells was quantified by real-time quantitative PCR.
The expression of TRPV1 and HSP70 in bone marrow cells was increased in rats with VH. Moxibustion at BL25 significantly decreased AWR scores and increased pain threshold pressure in rats with VH. Furthermore, moxibustion at BL25 significantly inhibited the VH-induced increase in the expression of TRPV1 and HSP70 in bone marrow cells.
The up-regulation of TRPV1 and HSP70 expression in bone marrow cells may be involved in visceral pain development and the analgesic effect of moxibustion on VH may be mediated through down-regulation of TRPV1 and HSP70 expression in bone marrow cells.
在大鼠内脏高敏（VH）模型中，研究艾灸对内脏高敏、骨髓细胞瞬时受体电位香草酸亚型1（TRPV1）及热休克蛋白（HSP）70表达的影响。
对新生斯普拉格-道利大鼠进行机械性结肠扩张以诱导内脏高敏。对8周龄的内脏高敏大鼠在穴位BL25或同侧非穴位处进行艾灸。在连续7天的艾灸治疗前后进行腹部退缩反射（AWR）评分和疼痛阈值压力评估。通过实时定量PCR对骨髓细胞中TRPV1和HSP70的表达进行定量分析。
内脏高敏大鼠骨髓细胞中TRPV1和HSP70的表达增加。艾灸BL25可显著降低内脏高敏大鼠的AWR评分，并提高疼痛阈值压力。此外，艾灸BL25可显著抑制内脏高敏诱导的骨髓细胞中TRPV1和HSP70表达的增加。
骨髓细胞中TRPV1和HSP70表达上调可能参与内脏痛的发生发展，艾灸对内脏高敏的镇痛作用可能是通过下调骨髓细胞中TRPV1和HSP70的表达来介导的。
Zotero 插件