Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
Stem Cells Int. 2015;2015:328957. doi: 10.1155/2015/328957. Epub 2015 Aug 3.
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.
雪旺细胞 (SC) 移植作为一种基于细胞的治疗方法，可以在实验中增强周围和中枢神经的修复，但由于供体部位发病率高，限制了其在临床应用中的应用。我们研究了从大鼠鼻中隔分离出的呼吸道黏膜干细胞 (REMSCs) 是否可以分化为功能性雪旺样细胞 (SC-like cells)。REMSCs 在体外快速增殖，并表达神经嵴标记物 (巢蛋白、波形蛋白、SOX10 和 CD44)。用神经胶质生长因子混合物处理 7 天后，REMSCs 分化为 SC-like 细胞。分化的 REMSCs(dREMSCs)呈现出类似于 SC 细胞的梭形形态。免疫细胞化学染色和 Western blot 表明 SC-like 细胞表达神经胶质标记物 (GFAP、S100β、Galc 和 P75) 和 CNPase。当与 dREMSCs 共培养 5 天时，PC12 细胞分化为具有长突起的成熟神经元样细胞。更重要的是，dREMSCs 可以在体外培养 21 天与 PC12 细胞的神经突形成髓鞘结构。我们的数据表明，REMSCs 是一种 EMSCs，可以分化为 SC-like 细胞，并且具有促进 PC12 细胞分化和形成髓鞘的能力。
