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中东呼吸综合征冠状病毒感染的生长与定量分析

Growth and Quantification of MERS-CoV Infection.

作者信息

Coleman Christopher M, Frieman Matthew B

机构信息

University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.

出版信息

Curr Protoc Microbiol. 2015 May 1;37(1):15E.2.1-9. doi: 10.1002/9780471729259.mc15e02s37.

DOI:10.1002/9780471729259.mc15e02s37
PMID:26344219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4735735/
Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.

摘要

中东呼吸综合征冠状病毒（MERS-CoV）是一种新出现的高致病性呼吸道病毒。尽管MERS-CoV在2012年才出现，但我们和其他研究人员已经开发出了在体外培养和定量感染性MERS-CoV及其复制RNA产物的检测方法。MERS-CoV能够感染多种细胞类型，但在Vero E6细胞中能以高滴度复制。本文介绍了MERS-CoV的繁殖和定量方法。

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[1]
Growth and Quantification of MERS-CoV Infection.

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[6]
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本文引用的文献

[1]
Infection with MERS-CoV causes lethal pneumonia in the common marmoset.

PLoS Pathog. 2014-8-21

[2]
Middle east respiratory syndrome coronavirus 4a protein is a double-stranded RNA-binding protein that suppresses PACT-induced activation of RIG-I and MDA5 in the innate antiviral response.

J Virol. 2014-2-12

[3]
The ORF4b-encoded accessory proteins of Middle East respiratory syndrome coronavirus and two related bat coronaviruses localize to the nucleus and inhibit innate immune signalling.

J Gen Virol. 2014-1-17

[4]
The structural and accessory proteins M, ORF 4a, ORF 4b, and ORF 5 of Middle East respiratory syndrome coronavirus (MERS-CoV) are potent interferon antagonists.

Protein Cell. 2013-12-8

[5]
Wild-type and innate immune-deficient mice are not susceptible to the Middle East respiratory syndrome coronavirus.

J Gen Virol. 2013-11-6

[6]
Middle East respiratory syndrome coronavirus (MERS-CoV) causes transient lower respiratory tract infection in rhesus macaques.

Proc Natl Acad Sci U S A. 2013-9-23

[7]
Middle East respiratory syndrome coronavirus accessory protein 4a is a type I interferon antagonist.

J Virol. 2013-9-11

[8]
The Middle East respiratory syndrome coronavirus (MERS-CoV) does not replicate in Syrian hamsters.

PLoS One. 2013-7-2

[9]
Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation.

J Infect Dis. 2013-3-26

[10]
Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans.

mBio. 2012-11-20

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