中东呼吸综合征冠状病毒感染的生长与定量分析
Growth and Quantification of MERS-CoV Infection.
作者信息
Coleman Christopher M, Frieman Matthew B
机构信息
University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.
出版信息
Curr Protoc Microbiol. 2015 May 1;37(1):15E.2.1-9. doi: 10.1002/9780471729259.mc15e02s37.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.
摘要
中东呼吸综合征冠状病毒（MERS-CoV）是一种新出现的高致病性呼吸道病毒。尽管MERS-CoV在2012年才出现，但我们和其他研究人员已经开发出了在体外培养和定量感染性MERS-CoV及其复制RNA产物的检测方法。MERS-CoV能够感染多种细胞类型，但在Vero E6细胞中能以高滴度复制。本文介绍了MERS-CoV的繁殖和定量方法。
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