Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Bioresour Technol. 2015 Dec;197:363-8. doi: 10.1016/j.biortech.2015.08.122. Epub 2015 Aug 31.
Nutrients are the deciding factors in the biological production of bioactive compounds. Various growth media like BG11, Zarrouk's and Chu's 10 were studied for carotenoid production in Synechocystis sp. CCNM 2501. Maximum carotenoid content (dry weight basis) was found in Zarrouk's medium (ZM, 7.99mgg(-1)) followed by BG11 (5.13mgg(-1)). Echinenone content was 4 times higher in ZM (3.81mgg(-1)) as compared to BG11 (0.95mgg(-1)) and Chu's 10 (0.77mgg(-1)). Being an economical medium, BG11 was selected for carotenoid production. Further, increase in salinity from 0 to 0.2M in BG11 medium increases total carotenoid content from 5.82 to 7.05mgg(-1) and later it declines to 6.23mgg(-1) (1M). 3 times more β-carotene is produced at 1M salinity as compared to control BG11. The variation in carotenoid composition with change in nutrients/salinity can be a good strategy to enhance certain targeted carotenoids.
营养素是生物活性化合物产生的决定性因素。研究了各种生长培养基，如 BG11、Zarrouk's 和 Chu's 10，以用于产生活性化合物的 Synechocystis sp. CCNM 2501。在 Zarrouk's 培养基（ZM，7.99mg/g）中发现最大的类胡萝卜素含量（干重基础），其次是 BG11（5.13mg/g）。与 BG11（0.95mg/g）和 Chu's 10（0.77mg/g）相比，ZM 中的echinenone 含量高 4 倍（3.81mg/g）。由于 BG11 是一种经济培养基，因此选择 BG11 用于生产类胡萝卜素。此外，BG11 培养基中盐度从 0 增加到 0.2M 时，总类胡萝卜素含量从 5.82 增加到 7.05mg/g，然后下降到 6.23mg/g（1M）。与对照 BG11 相比，1M 盐度下β-胡萝卜素的产量增加了 3 倍。随着营养物质/盐度的变化，类胡萝卜素组成的变化可能是增强某些靶向类胡萝卜素的良好策略。
