来自……的一种新型多糖的提取优化、结构分析及潜在生物活性

Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from .

作者信息

Shi He, Zhang Siyi, Zhu Mandi, Li Xiaoyan, Jie Weiguang, Kan Lianbao

机构信息

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.

School of Life Sciences, Northeast Forestry University, Harbin 150040, China.

出版信息

Antioxidants (Basel). 2024 Aug 8;13(8):965. doi: 10.3390/antiox13080965.

DOI:10.3390/antiox13080965

PMID:39199211

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11352142/

Abstract

is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of -1,6-D-Glc, -1,3-D-Glc, and -1,3,6-D-Glc linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.

摘要

是一种引起丝黑穗病的重要活体营养型病原菌。从患病高粱穗中提取的多糖具有显著的药用和食用价值。然而，这些新型多糖的结构和生物活性尚未得到研究。在本研究中，从的子实体中分离纯化出一种新型多糖（WM-NP'-60），旨在探索WM-NP'-60的结构特征以及显著的抗氧化和抗肿瘤特性。单糖组成测定、高碘酸氧化-史密斯降解、1D/2D-NMR分析和甲基化分析表明，WM-NP'-60主要由α-1,6-D-葡萄糖、β-1,3-D-葡萄糖和β-1,3,6-D-葡萄糖键组成。抗氧化试验表明，WM-NP'-60具有很强的活性，包括清除自由基、螯合亚铁离子和消除细胞内的活性氧（ROS）。通过透射电子显微镜（TEM）检测的HepG2、SGC7901和HCT116细胞显示出典型的凋亡小体。因此，本实验发现、提取并纯化了一种新型真菌多糖（WM-NP'-60），旨在为开发适合人类食用的新一代食品和营养保健品提供参考。