从菖蒲根茎中提取具有抗氧化和免疫活性的多糖的特性研究。
Characterization of polysaccharides with antioxidant and immunological activities from Rhizoma Acori Tatarinowii.
机构信息
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
出版信息
Carbohydr Polym. 2015 Nov 20;133:154-62. doi: 10.1016/j.carbpol.2015.07.018. Epub 2015 Jul 11.
Rhizoma Acori Tatarinowii is widely used in traditional Chinese medicine. In this study, three novel polysaccharides designated RATPW, RATPS1 and RATPS2 were isolated from Rhizoma Acori Tatarinowii by DEAE-52 cellulose chromatography. Their structures were characterized using physicochemical and spectral methods. Chemical analysis indicated that RATPW (6.5×10(3)Da) mainly composed of glucose and fructose. RATPS1 (1.5×10(5)Da) contained galactose and arabinose, while RATPS2 (5.3×10(4)Da) contained ∼49.5% galacturonic acid along with rhamnose, fructose, galactose, and arabinose. In vitro, RATPS2 showed the most significant scavenging activity on DPPH and hydroxyl radical. Three polysaccharides could protect the PC12 cells from H2O2-induced damage. Immunological tests indicated that both RATPW and RATPS2 significantly stimulated NO production and phagocytic activity in RAW264.7, and promoted splenocyte proliferation. These data suggested that polysaccharides RATPW and RATPS2 had the potential as novel natural sources of antioxidative and immunopotentiating agents.
菖蒲根茎在中医药中被广泛应用。在本研究中，我们通过 DEAE-52 纤维素层析从菖蒲根茎中分离得到三种新型多糖，分别命名为 RATPW、RATPS1 和 RATPS2。我们采用物理化学和光谱学方法对其结构进行了表征。化学分析表明，RATPW（6.5×10(3)Da）主要由葡萄糖和果糖组成。RATPS1（1.5×10(5)Da）含有半乳糖和阿拉伯糖，而 RATPS2（5.3×10(4)Da）含有约 49.5%的半乳糖醛酸以及鼠李糖、果糖、半乳糖和阿拉伯糖。体外实验表明，RATPS2 对 DPPH 和羟基自由基具有最强的清除活性。三种多糖均能保护 PC12 细胞免受 H2O2 诱导的损伤。免疫试验表明，RATPW 和 RATPS2 均能显著刺激 RAW264.7 细胞产生 NO 和吞噬活性，并促进脾细胞增殖。这些数据表明，多糖 RATPW 和 RATPS2 具有作为新型抗氧化和免疫增强剂天然来源的潜力。
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