College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
College of Resources, Sichuan Agricultural University, Chengdu, 611130, China.
Int J Biol Macromol. 2019 Jun 1;130:238-244. doi: 10.1016/j.ijbiomac.2019.02.119. Epub 2019 Feb 21.
Rhizome of the fern Dryopteris crassirhizoma Nakai is used as an antiviral drug in China. The objective was to characterize physicochemical properties, structural features and antioxidant and immunological activities of D. crassirhizoma polysaccharides. An acidic polysaccharide fraction (DCP-3) was obtained from Dryopteris crassirhizoma Nakai by purification with DEAE-52 and Sephadex G-100. DCP-3 was a novel triple-helical polysaccharide with an average MW of 273.2 kDa. This fraction was mainly composed of galactose (36.65%), xylose (34.75%), arabinose (17.07%) and mannose (9.22%). DCP-3 had strong activity for scavenging DPPH radical (IC: 2.04 mg/mL), hydroxyl radical (IC: 1.70 mg/mL), and superoxide anions (IC: 4.20 mg/mL) and also was capable of reducing ferric ions. In addition, nitric oxide production was enhanced in RAW264.7 macrophages stimulated by DCP-3. Based on these bioactivities, we inferred that DCP-3 was a functional component of D. crassirhizoma and may confer antivirus activity, with potential applications in functional food and drug industries.
中国将蕨类植物粗茎鳞毛蕨的根茎用作抗病毒药物。本研究旨在对粗茎鳞毛蕨多糖的理化性质、结构特征及抗氧化和免疫活性进行表征。通过 DEAE-52 和 Sephadex G-100 纯化，从粗茎鳞毛蕨中得到一种酸性多糖（DCP-3）。DCP-3 是一种新型的三螺旋多糖，平均分子量为 273.2 kDa。该级分主要由半乳糖（36.65%）、木糖（34.75%）、阿拉伯糖（17.07%）和甘露糖（9.22%）组成。DCP-3 对 DPPH 自由基（IC：2.04 mg/mL）、羟基自由基（IC：1.70 mg/mL）和超氧阴离子（IC：4.20 mg/mL）的清除活性均较强，并且能够还原铁离子。此外，DCP-3 还能增强 RAW264.7 巨噬细胞中一氧化氮的产生。基于这些生物活性，我们推断 DCP-3 是粗茎鳞毛蕨的功能成分，可能具有抗病毒活性，在功能性食品和药物行业具有潜在应用价值。
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