Niu Hong, Song Dan, Mu Haibo, Zhang Wuxia, Sun Feifei, Duan Jinyou
College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
Int J Biol Macromol. 2016 May;86:587-93. doi: 10.1016/j.ijbiomac.2016.01.111. Epub 2016 Feb 1.
Mushroom Inonotus obliquus (I. obliquus), a folk medicine, has been widely used to treat several human malicious tumors since 16th century. In this study, three homogenous biomolecules (designated IOA1, IOA2 and IOA3) were prepared from the alkali extract of I. obliquus. Their molecular weights were measured to be 6.1 × 10(4), 2.9 × 10(4) and 3.5 × 10(4) g/mol respectively and all of them were characterized as lignin-carbohydrate complexes mainly comprised lignin as well as -25% carbohydrates. Antioxidant assays indicated that all of them exhibited pronounced reductive power and strong scavenging activities on DPPH and hydroxyl radicals in vitro. Immunological tests showed that they could also significantly stimulate nitric oxide production and phagocytic activity in RAW 264.7 macrophages. These results implied that the lignin-carbohydrate complexes extracted from I. obliquus might be used as novel natural antioxidants or immunostimulants in functional foods or pharmaceutical candidates.
桦褐孔菌（Inonotus obliquus）是一种民间药物，自16世纪以来就被广泛用于治疗多种人类恶性肿瘤。在本研究中，从桦褐孔菌的碱提取物中制备了三种同质生物分子（命名为IOA1、IOA2和IOA3）。它们的分子量分别测定为6.1×10⁴、2.9×10⁴和3.5×10⁴ g/mol，并且所有这些分子都被表征为主要由木质素以及25%的碳水化合物组成的木质素-碳水化合物复合物。抗氧化试验表明，它们在体外均表现出显著的还原能力以及对DPPH和羟基自由基的强清除活性。免疫学测试表明，它们还可以显著刺激RAW 264.7巨噬细胞中一氧化氮的产生和吞噬活性。这些结果表明，从桦褐孔菌中提取的木质素-碳水化合物复合物可能用作功能性食品中的新型天然抗氧化剂或免疫刺激剂，或作为药物候选物。
