寄生于巴西东北部的吉米蟾蜍（Rhinella jimi）身上的圆角花蜱（Amblyomma rotundatum）中的贝利立克次体（Rickettsia bellii）
Rickettsia bellii in Amblyomma rotundatum ticks parasitizing Rhinella jimi from northeastern Brazil.
作者信息
Horta Mauricio C, Saraiva Danilo G, Oliveira Glauber M B, Martins Thiago F, Labruna Marcelo B
机构信息
University of São Francisco Valley, Univasf, Petrolina, PE, Brazil.
University of São Paulo, USP, São Paulo, SP, Brazil.
出版信息
Microbes Infect. 2015 Nov-Dec;17(11-12):856-8. doi: 10.1016/j.micinf.2015.08.010. Epub 2015 Sep 4.
This study evaluated rickettsial infection in Amblyomma rotundatum ticks collected from toads (Rhinella jimi) in the Brazilian Caatinga biome, an unique semiarid region of South America. Tick infestations were observed in 57.8% toads (26/45); mean infestation: 1.6 ticks/toad. DNA extraction from 42 ticks (6 larvae, 22 nymphs and 11 female adults) was tested by polymerase chain reaction (PCR) targeting Rickettsia organisms, which were detected in 100% of the ticks. Amplicons' DNA sequences were identical to each other and 99% identical to Rickettsia bellii from GenBank. DNA samples extracted from the blood of the 45 toads were negative by rickettsia-PCR protocols.
本研究评估了从巴西卡廷加生物群落的蟾蜍（Rhinella jimi）身上采集的圆角花蜱中的立克次体感染情况，卡廷加是南美洲一个独特的半干旱地区。在57.8%的蟾蜍（26/45）身上观察到蜱虫感染；平均感染率：1.6只蜱虫/蟾蜍。通过针对立克次体生物的聚合酶链反应（PCR）对从42只蜱虫（6只幼虫、22只若虫和11只成年雌蜱）中提取的DNA进行检测，在100%的蜱虫中检测到立克次体。扩增子的DNA序列彼此相同，与GenBank中的贝利立克次体有99%的同源性。通过立克次体PCR检测方案，从45只蟾蜍血液中提取的DNA样本均为阴性。
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