Su Wen, Wang Chengmin, Luo Jing, Zhao Yuliang, Wu Yan, Chen Lin, Zhao Na, Li Meng, Xing Chao, Liu Huimin, Zhang Hong, Chang Yung-fu, Li Tianxian, Ding Hua, Wan Xiufeng, He Hongxuan
National Research Center for Wildlife Born Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of the Chinese Academy of Sciences, Beijing 100101, China.
National Research Center for Wildlife Born Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Cell Rep. 2015 Sep 22;12(11):1831-41. doi: 10.1016/j.celrep.2015.08.029. Epub 2015 Sep 3.
Since 2013, avian influenza A(H7N9) viruses have diversified into multiple lineages by dynamically reassorting with other viruses, especially H9N2, in Chinese poultry. Despite concerns about the pandemic threat posed by H7N9 viruses, little is known about the biological properties of H7N9 viruses that may recruit internal genes from genetically distinct H9N2 viruses circulating among wild birds. Here, we generated 63 H7N9 reassortants derived from an avian H7N9 and a wild-bird-origin H9N2 virus. Compared with the wild-type parent, 25/63 reassortants had increased pathogenicity in mice. A reassortant containing PB1 of the H9N2 virus was highly lethal to mice and chickens but was not transmissible to guinea pigs by airborne routes; however, three substitutions associated with adaptation to mammals conferred airborne transmission to the virus. The emergence of the H7N9-pandemic reassortant virus highlights that continuous monitoring of H7N9 viruses is needed, especially at the domestic poultry/wild bird interface.
自2013年以来，甲型H7N9禽流感病毒通过与中国家禽中的其他病毒，尤其是H9N2病毒动态重配，分化成了多个谱系。尽管人们担心H7N9病毒构成的大流行威胁，但对于可能从在野生鸟类中传播的基因不同的H9N2病毒获取内部基因的H7N9病毒的生物学特性却知之甚少。在此，我们构建了63个源自禽H7N9病毒和野生鸟类源H9N2病毒的H7N9重配病毒。与野生型亲本相比，63个重配病毒中有25个在小鼠中的致病性增强。一个含有H9N2病毒PB1基因的重配病毒对小鼠和鸡具有高度致死性，但不能通过空气传播途径传染给豚鼠；然而，与适应哺乳动物相关的三个替换使该病毒获得了空气传播能力。H7N9大流行重配病毒的出现凸显了对H7N9病毒进行持续监测的必要性，尤其是在家禽/野生鸟类界面。
