Gabrielli Simona, Otašević Suzana, Ignjatović Aleksandra, Savić Sara, Fraulo Maurizio, Arsić-Arsenijević Valentina, Momčilović Stefan, Cancrini Gabriella
1 Department of Public Health and Infectious Diseases, "Sapienza" University of Rome , Rome, Italy .
2 Department of Microbiology and Immunology, Faculty of Medicine, University of Niš , Serbia, Niš, Serbia .
Vector Borne Zoonotic Dis. 2015 Sep;15(9):535-8. doi: 10.1089/vbz.2015.1797. Epub 2015 Sep 8.
During the years 2012-2014, a total of 158 outdoor dogs from Pančevo and Đurđevo (northern Serbia) and Niš and Prokuplje (southern Serbia) were submitted to molecular analyses (PCR and sequencing) for canine babesioses. An overall prevalence of 21.5% was found, due to the species Babesia sp. 'spanish dog' (10.1%), B. gibsoni (5.7%), B. canis vogeli (1.9%), B. caballi (1.9%), and B. microti (1.9%). In addition, sequence analysis showed the presence of Hepatozoon canis in a dog from Niš. No significant difference between infected and noninfected dogs was found by age, sex, and place of residence, whereas there was difference regarding the presence of ticks (p<0.005) and application of preventive measures such as applying of antitick drugs/devices. Moreover, a significant difference was established by area: Dogs from Prokuplje showed infection rates (59.1%) higher than dogs from Pančevo (11.9%), Niš (4.5), and Đurđevo (where infected dogs were not found), and a different geographical distribution of the species was found. The presence of so many Babesia species and the first identification of H. canis will allow investigations on the pathogenic role played by each one and suggests entomological studies on the tick species that are more suitable vectors for each of them. Finally, the presence of so many infected dogs offers the opportunity of evaluating the hypothesis of a possible zoonotic role of babesial species affecting dogs.
在2012年至2014年期间，来自潘切沃和久尔杰沃（塞尔维亚北部）以及尼什和普罗库普列（塞尔维亚南部）的总共158只户外犬被送去进行犬巴贝斯虫病的分子分析（聚合酶链反应和测序）。发现总体患病率为21.5%，病因是西班牙犬巴贝斯虫（10.1%）、吉氏巴贝斯虫（5.7%）、韦氏巴贝斯虫（1.9%）、马巴贝斯虫（1.9%）和微小巴贝斯虫（1.9%）。此外，序列分析显示来自尼什的一只犬体内存在犬肝簇虫。在感染犬和未感染犬之间，未发现年龄、性别和居住地方面的显著差异，而在蜱的存在情况（p<0.005）以及抗蜱药物/装置等预防措施的应用方面存在差异。此外，按地区确定了显著差异：来自普罗库普列的犬感染率（59.1%）高于来自潘切沃（11.9%）、尼什（4.5%）和久尔杰沃（未发现感染犬）的犬，并且发现了不同的物种地理分布。如此多巴贝斯虫物种的存在以及犬肝簇虫的首次鉴定将有助于研究每种物种所起的致病作用，并建议对更适合作为每种物种传播媒介的蜱种进行昆虫学研究。最后，如此多感染犬的存在提供了评估影响犬的巴贝斯虫物种可能具有人畜共患病作用这一假设的机会。
