Wang Ru, Zheng Ping, Zhang Meng, Zhao He-Ping, Ji Jun-Yuan, Zhou Xiao-Xin, Li Wei
Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
Bioresour Technol. 2015 Dec;197:410-5. doi: 10.1016/j.biortech.2015.08.135. Epub 2015 Sep 2.
Nitrate-dependent anaerobic ferrous oxidation (NAFO) is a new and valuable bio-process for the treatment of wastewaters with low C/N ratio, and the NAFO process is in state of the art. The heterotrophic denitrifying sludge (HDS), possessing NAFO activity, was used as bioaugmentation to enhance NAFO efficiency. At a dosage of 6% (V/V), the removal of nitrate and ferrous was 2.4 times and 2.3 times of as primary, and the volumetric removal rate (VRR) of nitrate and ferrous was 2.4 times and 2.2 times of as primary. Tracing experiments of HDS indicated that the bioaugmentation on NAFO reactor was resulted from the NAFO activity by HDS itself. The predominant bacteria in HDS were identified as Thauera (52.5%) and Hyphomicrobium (20.0%) which were typical denitrifying bacteria and had potential ability to oxidize ferrous. In conclusion, HDS could serve as bioaugmentation or a new seeding sludge for operating high-efficiency NAFO reactors.
硝酸盐依赖型厌氧亚铁氧化（NAFO）是一种用于处理低C/N比废水的新型且有价值的生物过程，并且NAFO工艺处于先进水平。具有NAFO活性的异养反硝化污泥（HDS）被用作生物强化剂以提高NAFO效率。在6%（V/V）的投加量下，硝酸盐和亚铁的去除量分别是初始值的2.4倍和2.3倍，硝酸盐和亚铁的容积去除率分别是初始值的2.4倍和2.2倍。HDS的追踪实验表明，NAFO反应器上的生物强化是由HDS自身的NAFO活性导致的。HDS中的优势菌被鉴定为陶厄氏菌（52.5%）和生丝微菌（20.0%），它们是典型的反硝化细菌，并且具有氧化亚铁的潜在能力。总之，HDS可作为生物强化剂或新型接种污泥用于运行高效NAFO反应器。
