Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, People's Republic of China,
J Ind Microbiol Biotechnol. 2014 May;41(5):803-9. doi: 10.1007/s10295-014-1424-5. Epub 2014 Mar 12.
Heterotrophic denitrifying enriched culture (DEC) from a lab-scale high-rate denitrifying reactor was discovered to perform nitrate-dependent anaerobic ferrous oxidation (NAFO). The DEC was systematically investigated to reveal their denitrification activity, their NAFO activity, and the predominant microbial population. The DEC was capable of heterotrophic denitrification with methanol as the electron donor, and autotrophic denitrification with ferrous salt as the electron donor named NAFO. The conversion ratios of ferrous-Fe and nitrate-N were 87.41 and 98.74 %, and the consumption Fe/N ratio was 2.3:1 (mol/mol). The maximum reaction velocity and half saturation constant of Fe were 412.54 mg/(l h) and 8,276.44 mg/l, and the counterparts of N were 20.87 mg/(l h) and 322.58 mg/l, respectively. The predominant bacteria were Hyphomicrobium, Thauera, and Flavobacterium, and the predominant archaea were Methanomethylovorans, Methanohalophilus, and Methanolobus. The discovery of NAFO by heterotrophic DEC is significant for the development of wastewater treatment and the biogeochemical iron cycle and nitrogen cycle.
从实验室规模的高速反硝化反应器中分离出的异养反硝化富集培养物（DEC）被发现具有硝酸盐依赖型厌氧亚铁氧化（NAFO）活性。本研究对 DEC 的反硝化活性、NAFO 活性及主要微生物种群进行了系统研究。DEC 能够以甲醇作为电子供体进行异养反硝化，也能够以亚铁盐作为电子供体进行自养反硝化，即 NAFO。亚铁-Fe 和硝酸盐-N 的转化率分别为 87.41%和 98.74%，消耗的 Fe/N 比为 2.3:1（摩尔/摩尔）。Fe 的最大反应速率和半饱和常数分别为 412.54mg/（l·h）和 8,276.44mg/l，N 的对应值分别为 20.87mg/（l·h）和 322.58mg/l。主要细菌为纤发菌属、陶厄氏菌属和黄杆菌属，主要古菌为产甲烷甲基杆菌属、甲烷盐杆菌属和甲醇球菌属。异养 DEC 具有 NAFO 活性，这对于废水处理以及生物地球化学铁循环和氮循环的发展具有重要意义。
