Ruan Yunjie, Taherzadeh Mohammad J, Kong Dedong, Lu Huifeng, Zhao Heping, Xu Xiangyang, Liu Yu, Cai Lei
Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Academy of Rural Development, Zhejiang University, Hangzhou 310058, China.
Microorganisms. 2020 Jan 2;8(1):72. doi: 10.3390/microorganisms8010072.
An aerobic denitrification strain, RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO-N, NO-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by , which was primarily performed in aerobic denitrification and cell assimilation, were conducted by and genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.
一株好氧反硝化菌株RAD - 17被鉴定出来，并且展现出高效的无机氮去除能力。在批次试验中，平均亚硝酸盐氮、硝酸盐氮和总铵氮（TAN）的去除率（去除效率>95%）分别为6.22 mg/(L∙h)、6.30 mg/(L∙h)和1.56 mg/(L∙h)。同时，通过单因素实验获得了最佳培养条件。对于氮去除途径，转录结果证明，由……编码的呼吸硝酸盐还原酶主要在好氧反硝化和细胞同化过程中发挥作用，这是由铵代谢的……基因进行的。此外，将菌株RAD - 17添加到实际废水中，其反硝化性能明显高于未接种组（84.22%对22.54%），并且在总细胞数的比例中，最大细胞丰度达到了28.5±4.5%。总体而言，高效的氮去除性能以及强大的环境适应性使得菌株RAD - 17成为循环水养殖系统（RAS）废水处理的潜在替代方案。 （注：原文中部分基因名称未给出具体内容，用“……”代替）
