Okonmah A D, Brown J W, Fishman L M, Carballeira A, Soliman K F
College of Pharmacy, Florida A. & M. University, Tallahassee.
Pharmacology. 1989;39(6):367-72. doi: 10.1159/000138624.
Cultured brain cells from rat fetuses of ethanol-treated mothers demonstrated more than 2-fold elevations in choline acetyltransferase (ChAT) activity relative to those of control (saline-exposed) fetal brain cells. When cells from control animals were incubated in vitro for 5 days with 0.1% ethanol, ChAT activity was found to increase more than 4-fold. Brain cells from in utero ethanol-treated animals further exposed to ethanol in vitro for 5 days demonstrated significantly higher ChAT activity compared to cells exposed to ethanol only in vivo. These levels were more than 6 times greater than those of central nervous system cells never exposed to ethanol. Acetylcholinesterase (AChE) activity was significantly elevated (greater than 4-fold) in fetal brain cells when ethanol was present both in vivo and in vitro, but neither treatment alone resulted in any significant changes in AChE. These effects of ethanol on enzymes involved in acetylcholine metabolism may contribute to the different developmental neurologic abnormalities associated with fetal alcohol exposure.
与对照组（暴露于生理盐水）胎儿脑细胞相比，来自乙醇处理过的母亲所产大鼠胎儿的培养脑细胞，其胆碱乙酰转移酶（ChAT）活性升高了两倍多。当将来自对照动物的细胞在体外与0.1%乙醇一起孵育5天时，发现ChAT活性增加了四倍多。与仅在体内暴露于乙醇的细胞相比，来自子宫内乙醇处理过的动物的脑细胞在体外进一步暴露于乙醇5天，其ChAT活性显著更高。这些水平比从未暴露于乙醇的中枢神经系统细胞高6倍多。当乙醇同时存在于体内和体外时，胎儿脑细胞中的乙酰胆碱酯酶（AChE）活性显著升高（超过四倍），但单独任何一种处理都不会导致AChE有任何显著变化。乙醇对参与乙酰胆碱代谢的酶的这些影响，可能导致与胎儿酒精暴露相关的不同发育性神经异常。
