Jha Bijay Kumar, Jung Hui-Jung, Seo Incheol, Suh Seong-Il, Suh Min-Ho, Baek Won-Ki
Department of Microbiology, Keimyung University School of Medicine, Daegu, Republic of Korea.
Department of Microbiology, Keimyung University School of Medicine, Daegu, Republic of Korea.
Exp Parasitol. 2015 Dec;159:100-6. doi: 10.1016/j.exppara.2015.09.005. Epub 2015 Sep 7.
Juglone (5-hydroxy-1,4-naphthoquinone) is a major chemical constituent of Juglans mandshruica Maxim. Recent studies have demonstrated that juglone exhibits anti-cancer, anti-bacterial, anti-viral, and anti-parasitic properties. However, its effect against Acanthamoeba has not been defined yet. The aim of this study was to investigate the effect of juglone on Acanthamoeba. We demonstrate that juglone significantly inhibits the growth of Acanthamoeba castellanii at 3-5 μM concentrations. Juglone increased the production of reactive oxygen species (ROS) and caused cell death of A. castellanii. Inhibition of ROS by antioxidant N-acetyl-l-cysteine (NAC) restored the cell viability. Furthermore, our results show that juglone increased the uptake of mitochondrial specific dye. Collectively, these results indicate that ROS played a significant role in the juglone-induced cell death of Acanthamoeba.
胡桃醌（5-羟基-1,4-萘醌）是胡桃楸的主要化学成分。最近的研究表明，胡桃醌具有抗癌、抗菌、抗病毒和抗寄生虫特性。然而，其对棘阿米巴的作用尚未明确。本研究的目的是探究胡桃醌对棘阿米巴的作用。我们证明，在3-5μM浓度下，胡桃醌能显著抑制卡氏棘阿米巴的生长。胡桃醌增加了活性氧（ROS）的产生，并导致卡氏棘阿米巴细胞死亡。抗氧化剂N-乙酰-L-半胱氨酸（NAC）对ROS的抑制作用恢复了细胞活力。此外，我们的结果表明，胡桃醌增加了线粒体特异性染料的摄取。总体而言，这些结果表明，ROS在胡桃醌诱导的棘阿米巴细胞死亡中起重要作用。
