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细柱五加(五加科)的完整叶绿体基因组

The complete chloroplast genome of Eleutherococcus gracilistylus (W.W.Sm.) S.Y.Hu (Araliaceae).

作者信息

Kim Kyunghee, Lee Junki, Lee Sang-Choon, Kim Nam-Hoon, Jang Woojong, Kim Soonok, Sung Sangmin, Lee Jungho, Yang Tae-Jin

机构信息

a Department of Plant Science , Plant Genomics and Breeding Institute , and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul , Republic of Korea .

b Phyzen Genomics Institute , Seoul , Republic of Korea .

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3741-2. doi: 10.3109/19401736.2015.1079884. Epub 2015 Sep 10.

Abstract

Eleutherococcus gracilistylus is a plant species that is close to E. senticosus, a famous medicinal plant called Siberian ginseng. The complete chloroplast genome sequence of the E. gracilistylus was determined by de novo assembly using whole genome next generation sequences. The chloroplast genome of E. gracilistylus was 156 770 bp long and showed distinct four partite structures such as a large single copy region of 86 729 bp, a small single copy region of 18 175 bp, and a pair of inverted repeat regions of 25 933 bp. The overall GC contents of the genome sequence were 36.8%. The chloroplast genome of E. gracilistylus contains 79 protein-coding sequences, 30 tRNA genes, and four rRNA genes. The phylogenetic analysis with the reported chloroplast genomes confirmed close taxonomical relationship of E. gracilistylus with E. senticosus.

摘要

细柱五加是一种与著名药用植物西伯利亚人参(刺五加)亲缘关系较近的植物物种。利用全基因组二代测序进行从头组装,测定了细柱五加的完整叶绿体基因组序列。细柱五加的叶绿体基因组长度为156770 bp,呈现出明显的四分体结构,即一个86729 bp的大单拷贝区域、一个18175 bp的小单拷贝区域和一对25933 bp的反向重复区域。基因组序列的总体GC含量为36.8%。细柱五加的叶绿体基因组包含79个蛋白质编码序列、30个tRNA基因和4个rRNA基因。与已报道的叶绿体基因组进行系统发育分析,证实了细柱五加与刺五加在分类学上的密切关系。

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