Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Bioresour Technol. 2015 Dec;198:77-86. doi: 10.1016/j.biortech.2015.08.139. Epub 2015 Sep 6.
Sodium sulfide (SS) was added to the non-detoxified wheat straw hydrolysate for ABE fermentation by Clostridium acetobutylicum CICC8012. Biochemical measurements demonstrated that supplementation with SS promoted earlier and enhanced conversion of acid to ABE and led to a 27.48% improvement in sugar consumption, a 20.48% improvement in the sugar-based ABE yield, a 47.63% improvement in the butanol titer, and a 53.50% improvement in the ABE concentration. The response of C. acetobutylicum CICC8012 at the mRNA level was examined by a transcriptional analysis performed with RNA sequencing. The expression of genes involved in the membrane transport of carbohydrates, glycolysis, and ABE formation increased following SS-supplemented fermentation, whereas the expression of genes encoding enzymes involved in acid formation decreased, which indicates that supplemental SS affected the central fermentative pathway, down-regulated the metabolic flux toward the acid formation branches, and up-regulated the metabolic flux toward the ABE formation branches.
硫化钠 (SS) 被添加到未经解毒的小麦秸秆水解物中，用于丙酮丁醇梭菌 CICC8012 的 ABE 发酵。生化测量表明，SS 的补充促进了酸向 ABE 的更早和更增强的转化，并导致糖消耗提高了 27.48%，基于糖的 ABE 产率提高了 20.48%，丁醇浓度提高了 47.63%，ABE 浓度提高了 53.50%。通过 RNA 测序进行的转录分析检查了 C. acetobutylicum CICC8012 在 mRNA 水平的反应。在 SS 补充发酵后，参与碳水化合物、糖酵解和 ABE 形成的膜转运的基因表达增加，而参与酸形成的酶编码基因的表达减少，这表明补充 SS 影响了中央发酵途径，下调了向酸形成分支的代谢通量，并上调了向 ABE 形成分支的代谢通量。
