Farrar Kerrie, Bryant David N, Turner Lesley, Gallagher Joe A, Thomas Ann, Farrell Markku, Humphreys Mervyn O, Donnison Iain S
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion SY23 3EB UK.
Bioenergy Res. 2012;5(1):149-157. doi: 10.1007/s12155-011-9156-0.
Increasing the extractable sugar yield from perennial crops is one strategy to generate renewable fuels such as bio-ethanol. L. (perennial ryegrass) can contain significant (>30% dry matter) water-soluble sugars in the form of polymeric fructan which is readily extracted, broken down and fermented to bio-ethanol. A population of generated from four parents which differed in water-soluble carbohydrate (WSC) content was subjected to multiple rounds of selection and recombination on the basis of early spring WSC content to produce a high WSC, and a low WSC population. A control population was generated by selecting the same number of plants at random. The alleles present at six candidate gene loci were analysed before and after selection and correlated to WSC content. Significant differences in the allele frequency of were observed between the three populations with one haplotype significantly associated with the high WSC C2 population (after three rounds of selection and two rounds of recombination). Moreover, WSC content was also associated with biomass accumulation. Thus, in addition to a 2.84-fold increase in WSC yield, the C2 population also had 1.48-fold more biomass per plant, resulting in 3.9-fold higher WSC yield per plant than the control population.
提高多年生作物的可提取糖产量是生产生物乙醇等可再生燃料的一种策略。多年生黑麦草（Lolium perenne L.）可以含有大量（>30%干物质）以聚合果聚糖形式存在的水溶性糖，这种糖很容易被提取、分解并发酵成生物乙醇。从四个水溶性碳水化合物（WSC）含量不同的亲本产生的一个群体，根据早春WSC含量进行多轮选择和重组，以产生一个高WSC群体和一个低WSC群体。通过随机选择相同数量的植株产生一个对照群体。在选择前后分析六个候选基因位点存在的等位基因，并将其与WSC含量相关联。在三个群体之间观察到等位基因频率的显著差异，其中一个单倍型与高WSC C2群体（经过三轮选择和两轮重组后）显著相关。此外，WSC含量也与生物量积累相关。因此，除了WSC产量增加2.84倍外，C2群体每株植物的生物量也多1.48倍，导致每株植物的WSC产量比对照群体高3.9倍。
