Barros Stephanie A, Chenoweth David M
Department of Chemistry , University of Pennsylvania , 231 South 34th Street , Philadelphia , PA 19104-6323 , USA . Email:
Chem Sci. 2015 Aug 14;6(8):4752-4755. doi: 10.1039/c5sc01595b. Epub 2015 Jun 10.
Nucleic acid three-way junctions (3WJs) play key roles in biological processes such as nucleic acid replication in addition to being implicated as dynamic transient intermediates in trinucleotide repeat sequences. Structural modulation of specific nucleic acid junctions could allow for control of biological processes and disease states at the nucleic acid level. Trinucleotide repeat expansions are associated with several neurodegenerative diseases where dynamic slippage is thought to occur during replication, forming transient 3WJ intermediates with the complementary strand. Here, we report triptycene-based molecules that bind to a d(CAG)·(CTG) repeat using a gel shift assay, fluorescence-quenching and circular dichroism.
核酸三向连接（3WJ）除了在三核苷酸重复序列中作为动态瞬时中间体外，在诸如核酸复制等生物过程中也起着关键作用。特定核酸连接的结构调节可以在核酸水平上控制生物过程和疾病状态。三核苷酸重复扩增与几种神经退行性疾病相关，据认为在复制过程中会发生动态滑移，与互补链形成瞬时3WJ中间体。在此，我们报告了基于三蝶烯的分子，其通过凝胶迁移试验、荧光猝灭和圆二色性与d（CAG）·（CTG）重复序列结合。
