三萜类小分子对大肠杆菌 rpoH 温度传感器的调控。
Modulation of the E. coli rpoH Temperature Sensor with Triptycene-Based Small Molecules.
机构信息
Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA, 19104, USA.
出版信息
Angew Chem Int Ed Engl. 2016 Jul 11;55(29):8258-61. doi: 10.1002/anie.201601626. Epub 2016 May 30.
Abstract
Regulation of the heat shock response (HSR) is essential in all living systems. In E. coli, the HSR is regulated by an alternative σ factor, σ(32) , which is encoded by the rpoH gene. The mRNA of rpoH adopts a complex secondary structure that is critical for the proper translation of the σ(32) protein. At low temperatures, the rpoH gene transcript forms a highly structured mRNA containing several three-way junctions, including a rare perfectly paired three-way junction (3WJ). This complex secondary structure serves as a primitive but highly effective strategy for the thermal control of gene expression. In this work, the first small-molecule modulators of the E. coli σ(32) mRNA temperature sensor are reported.
摘要
热休克反应 (HSR) 的调节对于所有生命系统都是至关重要的。在大肠杆菌中，HSR 由一个替代的 σ 因子 σ(32) 调节，该因子由 rpoH 基因编码。rpoH 的 mRNA 采用一种复杂的二级结构，这对于 σ(32) 蛋白的正确翻译至关重要。在低温下，rpoH 基因转录物形成一种高度结构化的 mRNA，其中包含几个三链结，包括一个罕见的完全配对的三链结 (3WJ)。这种复杂的二级结构是一种原始但非常有效的热控制基因表达的策略。在这项工作中，报告了第一个大肠杆菌 σ(32)mRNA 温度传感器的小分子调节剂。
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