Jia Li, Ding Lin, Tian Jiangwei, Bao Lei, Hu Yaoping, Ju Huangxian, Yu Jun-Sheng
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China.
Nanoscale. 2015 Oct 14;7(38):15953-61. doi: 10.1039/c5nr02224j. Epub 2015 Sep 14.
In this work we designed a MoS2 nanoplate-based nanoprobe for fluorescence imaging of intracellular ATP and photodynamic therapy (PDT) via ATP-mediated controllable release of (1)O2. The nanoprobe was prepared by simply assembling a chlorine e6 (Ce6) labelled ATP aptamer on MoS2 nanoplates, which have favorable biocompatibility, unusual surface-area-to-mass ratio, strong affinity to single-stranded DNA, and can quench the fluorescence of Ce6. After the nanoprobe was internalized into the cells and entered ATP-abundant lysosomes, its recognition to ATP led to the release of the single-stranded aptamer from MoS2 nanoplates and thus recovered the fluorescence of Ce6 at an excitation wavelength of 633 nm, which produced a highly sensitive and selective method for imaging of intracellular ATP. Meanwhile, the ATP-mediated release led to the generation of (1)O2 under 660 nm laser irradiation, which could induce tumor cell death with a lysosomal pathway. The controllable PDT provided a model approach for design of multifunctional theranostic nanoprobes. These results also promoted the development and application of MoS2 nanoplate-based platforms in biomedicine.
在这项工作中，我们设计了一种基于二硫化钼（MoS2）纳米片的纳米探针，用于通过ATP介导的单线态氧（1O2）可控释放实现细胞内ATP的荧光成像和光动力疗法（PDT）。该纳米探针通过简单地将氯e6（Ce6）标记的ATP适配体组装在MoS2纳米片上制备而成，MoS2纳米片具有良好的生物相容性、独特的表面积与质量比、对单链DNA的强亲和力，并且能够淬灭Ce6的荧光。纳米探针内化进入细胞并进入富含ATP的溶酶体后，其对ATP的识别导致单链适配体从MoS2纳米片上释放，从而在633 nm激发波长下恢复Ce6的荧光，这为细胞内ATP成像提供了一种高灵敏度和选择性的方法。同时，ATP介导的释放导致在660 nm激光照射下产生1O2，其可通过溶酶体途径诱导肿瘤细胞死亡。可控的光动力疗法为多功能诊疗纳米探针的设计提供了一种模式方法。这些结果也促进了基于MoS2纳米片的平台在生物医学中的开发和应用。
