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混合溶剂液相剥离的二硫化钼纳米颗粒作为过氧化物酶模拟物用于比色检测过氧化氢和葡萄糖。

Mixed-solvent liquid exfoliated MoS NPs as peroxidase mimetics for colorimetric detection of HO and glucose.

作者信息

Zhao Yuehua, Huang Yu, Wu Jiangling, Zhan Xiaoli, Xie Yuanyang, Tang Dongyun, Cao Haiyan, Yun Wen

机构信息

Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences Chongqing 400714 China

University of Chinese Academy of Science Beijing 100049 China.

出版信息

RSC Adv. 2018 Feb 15;8(13):7252-7259. doi: 10.1039/c7ra12584d. eCollection 2018 Feb 9.

DOI:10.1039/c7ra12584d
PMID:35540367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078403/
Abstract

Ultra-small molybdenum disulfide nanoparticles (MoS NPs) were prepared by a facile liquid exfoliation method with ethanol/water as the solvent. The produced MoS NPs were of high purity due to the easily removable ethanol/water solution. The prepared MoS NPs exhibited an intrinsic peroxidase-like activity in analogy to that of horseradish peroxidase (HRP). A custom-made spectrometer was employed to investigate the peroxidase-like activity of MoS NPs in the presence of HO and glucose. The change in absorption detected from MoS NPs is proportional to the amount of target. The calibration curve of HO and glucose shows a good relationship between the concentration of target and the change in the absorption of MoS NPs. The limit of detection of HO and glucose achieved by this method could approach 1.25 μM and 7 μM respectively. This method has been applied for the detection of glucose in serum from humans. Therefore, these produced MoS NPs offer an alternative high-efficiency and economic way to detect diabetes.

摘要

通过以乙醇/水为溶剂的简便液相剥离法制备了超小的二硫化钼纳米颗粒（MoS NPs）。由于乙醇/水溶液易于去除，所制备的MoS NPs具有高纯度。所制备的MoS NPs表现出类似于辣根过氧化物酶（HRP）的固有过氧化物酶样活性。使用定制的光谱仪研究了在过氧化氢（HO）和葡萄糖存在下MoS NPs的过氧化物酶样活性。从MoS NPs检测到的吸光度变化与靶标的量成正比。HO和葡萄糖的校准曲线显示了靶标浓度与MoS NPs吸光度变化之间的良好关系。通过该方法实现的HO和葡萄糖的检测限分别可接近1.25 μM和7 μM。该方法已应用于人体血清中葡萄糖的检测。因此，这些制备的MoS NPs为检测糖尿病提供了一种高效且经济的替代方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/7e11dbd0927a/c7ra12584d-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/a47f4be1d51d/c7ra12584d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/9bf0b3595f48/c7ra12584d-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/8b0bb043bc15/c7ra12584d-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/6b00b57b21c5/c7ra12584d-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/7e11dbd0927a/c7ra12584d-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/a47f4be1d51d/c7ra12584d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/9bf0b3595f48/c7ra12584d-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/8b0bb043bc15/c7ra12584d-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/6b00b57b21c5/c7ra12584d-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/9078403/7e11dbd0927a/c7ra12584d-f7.jpg

相似文献

[1]
Mixed-solvent liquid exfoliated MoS NPs as peroxidase mimetics for colorimetric detection of HO and glucose.

RSC Adv. 2018-2-15

[2]
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[3]
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[4]
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[5]
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Spectrochim Acta A Mol Biomol Spectrosc. 2021-10-5

[6]
Enhancing sensitivity and selectivity in a label-free colorimetric sensor for detection of iron(II) ions with luminescent molybdenum disulfide nanosheet-based peroxidase mimetics.

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[7]
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J Colloid Interface Sci. 2020-10-15

[8]
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[9]
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[10]
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引用本文的文献

[1]
Iron-Coordinated L-Lysine-Based Nanozymes with High Peroxidase-like Activity for Sensitive Hydrogen Peroxide and Glucose Detection.

Polymers (Basel). 2023-7-10

[2]
Enzyme/Nanocopper Hybrid Nanozymes: Modulating Enzyme-like Activity by the Protein Structure for Biosensing and Tumor Catalytic Therapy.

ACS Appl Mater Interfaces. 2021-2-3

[3]
2D CTAB-MoSe Nanosheets and 0D MoSe Quantum Dots: Facile Top-Down Preparations and Their Peroxidase-Like Catalytic Activity for Colorimetric Detection of Hydrogen Peroxide.

Nanomaterials (Basel). 2020-10-16

[4]
Polyethyleneimine-Stabilized Platinum Nanoparticles as Peroxidase Mimic for Colorimetric Detection of Glucose.

ACS Omega. 2020-3-20

本文引用的文献

[1]
Aptamer loaded MoS2 nanoplates as nanoprobes for detection of intracellular ATP and controllable photodynamic therapy.

Nanoscale. 2015-10-14

[2]
SDS-MoS2 nanoparticles as highly-efficient peroxidase mimetics for colorimetric detection of H2O2 and glucose.

Talanta. 2015-8-15

[3]
Two-dimensional transition metal dichalcogenide (TMD) nanosheets.

Chem Soc Rev. 2015-5-7

[4]
Hemin-functionalized WS2 nanosheets as highly active peroxidase mimetics for label-free colorimetric detection of H2O2 and glucose.

Analyst. 2015-4-21

[5]
A colorimetric method of analysis for trace amounts of hydrogen peroxide with the use of the nano-properties of molybdenum disulfide.

Analyst. 2015-2-21

[6]
Seeing diabetes: visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2 nanosheets.

Nanoscale. 2014-10-21

[7]
Polypyrrole nanoparticles as promising enzyme mimics for sensitive hydrogen peroxide detection.

Chem Commun (Camb). 2014-3-21

[8]
Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose.

Talanta. 2013-12-19

[9]
MIL-53(Fe): a metal-organic framework with intrinsic peroxidase-like catalytic activity for colorimetric biosensing.

Chemistry. 2013-11-4

[10]
TiO(2) nanotube arrays: intrinsic peroxidase mimetics.

Chem Commun (Camb). 2013-11-18

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