Sanz Martín, Picazo-Bueno José Angel, García Javier, Micó Vicente
Opt Express. 2015 Aug 10;23(16):21352-65. doi: 10.1364/OE.23.021352.
We report on a novel algorithm for high-resolution quantitative phase imaging in a new concept of lensless holographic microscope based on single-shot multi-wavelength illumination. This new microscope layout, reported by Noom et al. along the past year and named by us as MISHELF (initials incoming from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel) microscopy, rises from the simultaneous illumination and recording of multiple diffraction patterns in the Fresnel domain. In combination with a novel and fast iterative phase retrieval algorithm, MISHELF microscopy is capable of high-resolution (micron range) phase-retrieved (twin image elimination) biological imaging of dynamic events. In this contribution, MISHELF microscopy is demonstrated through qualitative concept description, algorithm implementation, and experimental validation using both a synthetic object (resolution test target) and a biological sample (swine sperm sample) for the case of three (RGB) illumination wavelengths. The proposed method becomes in an alternative instrument improving the capabilities of existing lensless microscopes.
我们报道了一种基于单次多波长照明的无透镜全息显微镜新概念下的高分辨率定量相位成像新算法。这种新的显微镜布局由诺姆等人在过去一年报道，我们将其命名为MISHELF（来自多照明单全息曝光无透镜菲涅耳的首字母缩写）显微镜，它源于在菲涅耳域中同时照明和记录多个衍射图案。结合一种新颖且快速的迭代相位检索算法，MISHELF显微镜能够对动态事件进行高分辨率（微米范围）的相位检索（消除孪生图像）生物成像。在本论文中，通过定性概念描述、算法实现以及使用合成物体（分辨率测试靶）和生物样本（猪精子样本）对三种（RGB）照明波长情况进行的实验验证，展示了MISHELF显微镜。所提出的方法成为一种改进现有无透镜显微镜功能的替代仪器。
