配备有组织的3D干细胞的双隔室和混合植入物，用于骨软骨再生纳米医学。

Double compartmented and hybrid implant outfitted with well-organized 3D stem cells for osteochondral regenerative nanomedicine.

作者信息

Keller Laetitia, Wagner Quentin, Schwinté Pascale, Benkirane-Jessel Nadia

机构信息

INSERM UMR1109, Osteoarticular & Dental Regenerative Nanomedicine, Faculté de Médecine, FMTS, F-67085 Strasbourg, France.

Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.

出版信息

Nanomedicine (Lond). 2015;10(18):2833-45. doi: 10.2217/nnm.15.113. Epub 2015 Sep 7.

DOI:10.2217/nnm.15.113

PMID:26377156

Abstract

AIM

Articular cartilage repair remains challenging, because most clinical failures are due to the lack of subchondral bone regeneration. We report an innovative approach improving cartilage repair by regenerating a robust subchondral bone, supporting articular cartilage.

MATERIALS & METHODS: We developed a compartmented living implant containing triple-3D structure: stem cells as microtissues for embryonic endochondral development mimic, nanofibrous collagen to enhance mineralization for subchondral bone and alginate hydrogel for cartilage regeneration.

RESULTS & CONCLUSION: This system mimics the natural gradient of the osteochondral unit, using only one kind of stem cell, targeting their ability to express specific bone or cartilage proteins. Mineralization gradient of articular cartilage and the natural 'glue' between subchondral bone and cartilage were reproduced in vitro.

摘要