用于软骨组织工程的纳米结构胶囊

Nanostructured Capsules for Cartilage Tissue Engineering.

作者信息

Correia Clara R, Reis Rui L, Mano João F

机构信息

3B' s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal.

ICVS/3B's-PT Government Associate Laboratory, Guimarães, Braga, Portugal.

出版信息

Methods Mol Biol. 2015;1340:181-9. doi: 10.1007/978-1-4939-2938-2_13.

DOI:10.1007/978-1-4939-2938-2_13

PMID:26445839

Abstract

Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(L-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(L-lactic acid) microparticles for cartilage tissue engineering.

摘要

聚合物多层胶囊（PMCs）在生物封装领域有广泛应用，生物封装是组织工程和再生医学中一个不断发展的分支。在此，我们描述了由聚（L-赖氨酸）、海藻酸盐和壳聚糖通过逐层组装形成的外部多层膜组成的分级PMCs的制备。PMCs的核心被液化，并封装了用于软骨组织工程的人脂肪干细胞和表面功能化的胶原II-转化生长因子-β3聚（L-乳酸）微粒。