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苜蓿中华根瘤菌4H41菌株的高质量永久草图基因组序列,该菌株是菜豆一种有效的耐盐耐旱微共生体。

High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris.

作者信息

Mhamdi Ridha, Ardley Julie, Tian Rui, Seshadri Rekha, Reddy T B K, Pati Amrita, Woyke Tanja, Markowitz Victor, Ivanova Natalia, Kyrpides Nikos, Reeve Wayne

机构信息

Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, BP 901, Hammam-Lif, 2050 Tunisia.

Centre for Rhizobium Studies, Murdoch University, Murdoch, WA Australia.

出版信息

Stand Genomic Sci. 2015 Jul 2;10:34. doi: 10.1186/s40793-015-0005-1. eCollection 2015.

Abstract

Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.

摘要

苜蓿中华根瘤菌4H41是一种需氧、能运动、革兰氏阴性、不形成芽孢的杆菌,它既可以作为土壤腐生菌存在,也可以作为普通菜豆(Phaseolus vulgaris)的豆科微共生体存在。菌株4H41于2002年从突尼斯南部Rjim-Maatoug绿洲种植的普通菜豆根瘤中分离得到。菌株4H41耐盐耐旱,与普通菜豆共生固氮效率高。在此,我们描述了苜蓿中华根瘤菌4H41的特征,以及基因组序列信息及其注释。这个6,795,637 bp的高质量永久草图基因组被排列成47个支架,由47个重叠群组成,包含6,350个蛋白质编码基因和72个仅编码RNA的基因,它是作为美国能源部联合基因组研究所2010年细菌和古菌基因组百科全书-根瘤菌(GEBA-RNB)项目提案的一部分而测序的根瘤菌基因组之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec5/4571125/a91a8a499b00/40793_2015_5_Fig1_HTML.jpg

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