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从印度塔尔沙漠本土豆科树木(牧豆树)的固氮根瘤中分离出的Ensifer sp. PC2的高质量永久草图基因组序列。

High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India.

作者信息

Gehlot Hukam Singh, Ardley Julie, Tak Nisha, Tian Rui, Poonar Neetu, Meghwal Raju R, Rathi Sonam, Tiwari Ravi, Adnawani Wan, Seshadri Rekha, Reddy T B K, Pati Amrita, Woyke Tanja, Pillay Manoj, Markowitz Victor, Baeshen Mohammed N, Al-Hejin Ahmed M, Ivanova Natalia, Kyrpides Nikos, Reeve Wayne

机构信息

BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.

Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.

出版信息

Stand Genomic Sci. 2016 Jun 23;11:43. doi: 10.1186/s40793-016-0157-7. eCollection 2016.

Abstract

Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.

摘要

Ensifer sp. PC2是一种需氧、具运动性、革兰氏阴性、不形成芽孢的杆菌,它是从豆科树木灰毛叶豆(P. cineraria (L.) Druce (Khejri))的固氮根瘤中分离得到的,灰毛叶豆是生长在印度塔尔沙漠干旱和半干旱地区的一种关键物种。PC2菌株在拉贾斯坦邦西部的碱性土壤中作为优势腐生菌存在。它生长迅速,能很好地适应干旱条件,并且能够与几种一年生豆科作物以及含羞草科树木和灌木形成有效的共生关系。在此,我们描述了Ensifer sp. PC2的特征,以及基因组序列信息及其注释。这个8,458,965 bp的高质量永久草图基因组被组装成171个重叠群的171个支架,包含8,344个蛋白质编码基因和139个仅编码RNA的基因,它是作为美国能源部联合基因组研究所2010年细菌和古菌基因组百科全书 - 根瘤菌(GEBA - RNB)项目提案的一部分进行测序的根瘤菌基因组之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67a1/4918122/d341147a60fe/40793_2016_157_Fig1_HTML.jpg

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