Perez-Casal Jose, Prysliak Tracy, Maina Teresa, Wang Yejun, Townsend Hugh, Berverov Emil, Nkando Isabel, Wesonga Hezron, Liljander Anne, Jores Joerg, Naessens Jan, Gerdts Volker, Potter Andrew
Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
Vet Immunol Immunopathol. 2015 Nov 15;168(1-2):103-10. doi: 10.1016/j.vetimm.2015.08.013. Epub 2015 Sep 9.
Current contagious bovine pleuropneumonia (CBPP) vaccines are based on live-attenuated strains of Mycoplasma mycoides subsp. mycoides (Mmm). These vaccines have shortcomings in terms of efficacy, duration of immunity and in some cases show severe side effects at the inoculation site; hence the need to develop new vaccines to combat the disease. Reverse vaccinology approaches were used and identified 66 candidate Mycoplasma proteins using available Mmm genome data. These proteins were ranked by their ability to be recognized by serum from CBPP-positive cattle and thereafter used to inoculate naïve cattle. We report here the inoculation of cattle with recombinant proteins and the subsequent humoral and T-cell-mediated immune responses to these proteins and conclude that a subset of these proteins are candidate molecules for recombinant protein-based subunit vaccines for CBPP control.
目前的传染性牛胸膜肺炎（CBPP）疫苗是基于减毒活的丝状支原体丝状亚种（Mmm）菌株。这些疫苗在效力、免疫持续时间方面存在不足，并且在某些情况下在接种部位会出现严重的副作用；因此需要开发新的疫苗来对抗这种疾病。采用反向疫苗学方法，利用现有的丝状支原体基因组数据鉴定出66种候选支原体蛋白。根据CBPP阳性牛血清识别这些蛋白的能力对其进行排名，然后用于接种未感染的牛。我们在此报告用重组蛋白接种牛以及随后对这些蛋白的体液免疫和T细胞介导的免疫反应，并得出结论，这些蛋白中的一部分是用于基于重组蛋白的亚单位疫苗以控制CBPP的候选分子。
