Valente Cecília Souza, Alfieri Alice Fernandes, Barry Aline Fernandes, Leme Raquel Arruda, Lorenzetti Elis, Alfieri Amauri Alcindo
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
Trop Anim Health Prod. 2016 Jan;48(1):21-7. doi: 10.1007/s11250-015-0912-6. Epub 2015 Sep 18.
This study aimed to evaluate the natural infection by SaV in pigs of different categories of production cycle in an important Brazilian pig-producing region. Faecal samples (n = 169) of suckling, post-weaning, finisher and breeder pig categories were analysed. Animals were from five farrow-to-weaning and nine grower-to-finish commercial pig farms. The RT-PCR assay was performed targeting the partial RNA-dependent RNA polymerase (RdRp) gene of porcine SaV genome. The virus was detected in 23.7% (40/169) of faecal samples and in 10/14 (5/5 farrow-to-weaning; 5/9 grower-to-finish) of pig farms evaluated. Porcine SaV was most frequently (p < 0.05) detected in pigs at post-weaning than in grower-to-finish and breeder categories. The phylogenetic analysis revealed that the porcine SaV strains belong to the GIII and GIX? genogroups. This study showed that the porcine SaV GIII genogroup has spread in the pig herds and provides the first evidence of GIX? genogroup circulation in South America.
本研究旨在评估巴西一个重要养猪地区不同生产周期阶段猪群中猪萨波病毒（SaV）的自然感染情况。分析了哺乳仔猪、断奶仔猪、育肥猪和种猪的粪便样本（n = 169）。动物来自5个从产仔到断奶以及9个从生长到育肥的商业猪场。采用逆转录聚合酶链反应（RT-PCR）检测方法，针对猪SaV基因组的部分依赖RNA的RNA聚合酶（RdRp）基因进行检测。在23.7%（40/169）的粪便样本中检测到该病毒，在所评估的14个猪场中有10个（5个从产仔到断奶的猪场；5个从生长到育肥的猪场）检测到该病毒。与育肥猪和种猪相比，断奶仔猪中猪SaV的检出频率最高（p < 0.05）。系统发育分析表明，猪SaV毒株属于GIII和GIX? 基因群。本研究表明猪SaV GIII基因群已在猪群中传播，并首次提供了GIX? 基因群在南美洲传播的证据。
