Liu Xiao, Song Chunlian, Liu Yinghua, Qu Kaixing, Bi Junyu, Bi Junlong, Wang Yunhua, Yang Ying, Sun Junhua, Guo Zhigang, Li Ganwu, Liu Jianping, Yin Gefen
College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
Academy of Science and Technology, Chuxiong Normal University, Chuxiong, China.
Front Vet Sci. 2022 Jul 7;9:854905. doi: 10.3389/fvets.2022.854905. eCollection 2022.
As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV-GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in region in 10 of the 20 GIII strains, as well as deletions and insertions in region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China.
作为最重要的肠道病毒之一，札如病毒（SaV）可感染人类和多种动物。截至目前，已鉴定出19个SaV基因组群，其中4个来自人类（GI、GII、GIV和GV），8个来自猪（GIII、GV - GXI）。猪札如病毒（PoSaV）GIII在中国一直流行；然而，云南省PoSaV感染状况仍不清楚。在本研究中，于2020年1月至5月期间从云南省与急性腹泻暴发相关的仔猪中收集了202份粪便样本。PoSaV检测显示，云南省PoSaV总感染率为35.2%，确定了21株PoSaV毒株并进行了系统发育分析。系统发育树分析表明，20株PoSaV毒株属于GIII，分为五个基因型，而一株PoSaV毒株（YNQB）属于GV。序列比对显示，20株GIII毒株中有10株在 区域存在缺失，GV毒株（YNQB）在 区域存在缺失和插入。此外，基因组重组分析表明，两株GIII毒株（YNAN和YNJD）是重组体，分别与参考序列MK965898和LC215880、MK965898和FJ387164密切相关。总之，2020年在云南省鉴定出PoSaV - GIII毒株，首次从中国鉴定出一株PoSaV - GV毒株，而综合分析表明云南省PoSaV毒株具有高度遗传多样性。本研究可能为云南省当前的PoSaV感染提供新的见解，并为中国进一步控制PoSaV感染铺平道路。
