巴西猪群自然感染的戊型肝炎病毒的分子检测和特征分析。
Molecular detection and characterization of hepatitis E virus in naturally infected pigs from Brazilian herds.
机构信息
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990 Londrina, Parana, Brazil.
出版信息
Res Vet Sci. 2012 Dec;93(3):1515-9. doi: 10.1016/j.rvsc.2012.06.003. Epub 2012 Jul 4.
The aim of this study was to investigate the presence of swine hepatitis E virus (HEV) from pigs of different production categories and from different pig farms in South Brazil. A total of 170 porcine faecal samples from breeder sows, boars, suckling piglets, weaned and growing pigs were collected from 14 pig farms. The faecal samples were screened by nested RT-PCR using primers targeting the ORF2 region of HEV genome. The samples that were positive from this screening were used in a nested RT-PCR targeting the ORF1 region. The screening detected HEV RNA in 62.5% of the pig farms and in 15.3% of the faecal samples. In 15 faecal samples, it was possible to amplify the HEV RNA with both the ORF1 and ORF2 regions. The phylogenetic analyses obtained for both ORFs confirmed that all of the Brazilian swine HEV isolates clustered with genotype 3b, the same genotype described previously in humans in Brazil.
本研究旨在调查巴西南部不同生产类别的猪和不同养猪场猪群中猪戊型肝炎病毒（HEV）的存在情况。从 14 个养猪场采集了 170 份母猪、公猪、哺乳仔猪、断奶仔猪和生长猪的粪便样本。采用针对 HEV 基因组 ORF2 区的巢式 RT-PCR 对粪便样本进行筛查。对筛查呈阳性的样本，采用针对 ORF1 区的巢式 RT-PCR 进行检测。筛查检测到 62.5%的养猪场和 15.3%的粪便样本中存在 HEV RNA。在 15 份粪便样本中，能够同时扩增出 ORF1 和 ORF2 区的 HEV RNA。对这两个 ORF 进行的系统进化分析证实，所有巴西猪 HEV 分离株与基因型 3b 聚类，与之前在巴西人类中描述的相同基因型。
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