用于制备热稳定生物基材料的寡聚（L-半胱氨酸）的化学酶法合成
Chemoenzymatic Synthesis of Oligo(L-cysteine) for Use as a Thermostable Bio-Based Material.
作者信息
Ma Yinan, Sato Ryota, Li Zhibo, Numata Keiji
机构信息
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Enzyme Research Team, Biomass Engineering Program Cooperation Division, Center for Sustainable Resource Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
出版信息
Macromol Biosci. 2016 Jan;16(1):151-9. doi: 10.1002/mabi.201500255. Epub 2015 Sep 21.
Oligomerization of thiol-unprotected L-cysteine ethyl ester (Cys-OEt) catalyzed by proteinase K in aqueous solution has been used to synthesize oligo(L-cysteine) (OligoCys) with a well-defined chemical structure and relatively large degree of polymerization (DP) up to 16-17 (average 8.8). By using a high concentration of Cys-OEt, 78.0% free thiol content was achieved. The thermal properties of OligoCys are stable, with no glass transition until 200 °C, and the decomposition temperature could be increased by oxidation. Chemoenzymatically synthesized OligoCys has great potential for use as a thermostable bio-based material with resistance to oxidation.
在水溶液中，蛋白酶K催化的硫醇未保护的L-半胱氨酸乙酯（Cys-OEt）低聚反应已被用于合成具有明确化学结构和相对较大聚合度（DP）高达16 - 17（平均8.8）的聚（L-半胱氨酸）（OligoCys）。通过使用高浓度的Cys-OEt，实现了78.0%的游离硫醇含量。OligoCys的热性能稳定，直到200°C才出现玻璃化转变，并且分解温度可通过氧化提高。化学酶法合成的OligoCys作为一种抗氧化的热稳定生物基材料具有巨大的应用潜力。
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