Chen Fangfang, Zhao Weifeng, Zhang Jingjing, Kong Jie
MOE Key Laboratory of Space Applied Physics and Chemistry, Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, 710072, P. R. China.
Phys Chem Chem Phys. 2016 Jan 14;18(2):718-25. doi: 10.1039/c5cp04218f.
Surface molecular imprinting for proteins is an emerging cross-field of molecular imprinting engineering and functional materials. In this contribution, we report a novel design of magnetic two-dimensional molecularly imprinted polymers (2D-MIPs) for the high recognition and separation of proteins. Bovine serum albumin-surface-imprinted polydopamines were prepared on the surfaces of the magnetic Fe3O4-graphene oxide hybrid to form magnetic 2D-MIPs for proteins. The 2D Fe3O4-graphene oxide substrate possesses a dominant surface-to-volume ratio in comparison to 3D spherical substrates with the same volume. These materials are sensitive to a magnetic field and can be easily separated using an external magnet. The binding experimental results of bovine serum albumin on magnetic 2D-MIPs and real sample analysis demonstrated the high recognition specificity, selectivity, accessibility and convenient separation of 2D-MIPs for template protein. The design and synthesis of magnetic 2D-MIPs provide a new perspective for the surface molecularly imprinted materials with potential in the recognition and separation of proteins.
蛋白质的表面分子印迹是分子印迹工程与功能材料领域中一个新兴的交叉领域。在本论文中，我们报道了一种用于蛋白质高效识别与分离的磁性二维分子印迹聚合物（2D-MIPs）的新颖设计。在磁性Fe3O4-氧化石墨烯复合材料表面制备了牛血清白蛋白表面印迹聚多巴胺，以形成用于蛋白质的磁性二维分子印迹聚合物。与具有相同体积的三维球形基质相比，二维Fe3O4-氧化石墨烯基质具有显著的表面积与体积比。这些材料对磁场敏感，可通过外部磁铁轻松分离。牛血清白蛋白在磁性二维分子印迹聚合物上的结合实验结果及实际样品分析表明，二维分子印迹聚合物对模板蛋白具有高识别特异性、选择性、可及性和便捷的分离性能。磁性二维分子印迹聚合物的设计与合成，为具有蛋白质识别与分离潜力的表面分子印迹材料提供了新的视角。
